Why is MSX not working?

Melis Kuskucu Korkut @Melis-Kuskucu-Korkut

08 August 2024 1 5K Report

Hi everyone. I am trying to perform the selection process of the transfected GS-deficient CHO cells with MSX, I use 50 uM MSX which should be more than enough but cells have still above 90% viability after 3-4 passages and titer is still low. Do you have any idea about what happens?

Alejandro Martin

Melis Kuskucu Korkut exactly what CHO line are you using? Is this a knockout? Also, what medium? Are you supplementing the medium with MSX right before use or are you preparing a stock that is then refrigerated and stored before use?

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