Hi All,

I have been trying to perform RNAscope in whole mount retina of mice at postnatal day 6. Since there are a lot of incubation and washing steps, I need to transfer retina between 2 mL microcentrifuge tubes and 96 well plate. This is leading to breakdown of the retina tissue. I have tried transferring the dissected retina using a Pasteur pipette or wide cut 1 mL pipette tip but still there is disruption of retina tissue.

Can anyone suggest me a way to overcome this problem?

Thanks in advance for any suggestions.