I am currently cloning my genes and i am short on TA vector if i want to clone TA vector and increase it copy. After isolating these vectors, will it retain its T overhangs?
The TA cloning relies on A overhangs that are added nonspecifically by a DNA polymerase such as Taq to PCR fragments and T overhangs that are added to the plasmid (must be prepared separately). Combining these two, you get better ligation efficiency than using blunt ends.
So, the way to prepare a vector for TA cloning is to cut is with an enzyme that makes blunt ends (e.g. SmaI), then add Taq, buffer and ONLY dTTP. The polymerase will add it at the 3' ends of the linear DNA fragments. When the polymerase has all dNTP to chose from, it adds preferentially dATP at the 3' ends. In this way, you get complementary DNA fragments (your PCR with A overhangs, your vector with T overhangs) that can be ligated. Happy cloning :-)