I'm trying to differentiate SH-SY5Y cells but they're not responding as expected. Is there a specific method for diluting RA?

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Malcolm Nobre

Hello Mahima Mishra

May be you are not following the right protocol. The paper attached below will be helpful!

Article Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

Best,

Mahima Mishra

Hi Malcolm Nobre

I am using the same protocol but the cells get dead after 10 days and detach from the surface. Could you please suggest here?