I am doing a research with anaplasma sp. And I have infected my cell line with anaplasma possitive blood RBC and after 1-2 days of incubation an odor is produced in the incubator.
As a former PhD scholar, currently working as a Junior Research Fellow on a DST-funded project, I am seeking information on travel grants. A few months ago, while I was still pursuing my PhD, I...
03 February 2024 2,050 0 View
Trying to find the relationship between epigenetic and cancer more deeply.
30 December 2023 8,363 2 View
Hello all , I have a two-plate configuration and there is a dielectric in between them . I launch electrons from plate and it reaches another plates through the dielectric . I kept the dielectric...
16 October 2023 5,702 0 View
Hello, I have performed MM-PBSA calculation of a protein-ligand complex. I utilised approx 750 frames (out of a total of 15000 frames) to compute the free enrgy change of binding. Then, I used a...
04 October 2023 247 2 View
primers are short oligonucleotides specifically designed for sequencing and PCR. But if we don't know where to start sequencing for a novel virus's genome then how the primers can be designed? or...
19 September 2023 4,636 3 View
Cancer means the uncontrol cell division, but what factors increase the risk of breast cancer in females other than the genetic issue?
18 September 2023 5,790 4 View
How can I pick up the microplastic particle when doing FTIR-ATR? Beacuse I can not directly place my filter paper under ATR.
01 September 2023 2,004 9 View
I am starting my CHIP qPCR on MCF7 and MDAmb231 cell lines and I am Using the IMPRINT CHIP kit by Sigma. I need to optimize the sonication time for both cell lines, but the buffers are very...
03 August 2023 3,170 0 View
I have a comparative transcriptome dataset, with a number of upregulated and downregulated genes. I want to construct a protein-protein interactome network to understand the node and hub genes and...
23 July 2023 3,286 0 View
Although there are several research papers describing methods to estimate different organic phosphorus fractions, I did not found any comprehensive method to estimate different organic P...
25 June 2023 6,228 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View