1. Is recombinant TGF-β1 protein widely utilized to establish in vitro and in vivo fibrosis models across different tissues (such as lung, liver, kidney, or skin)?
2. What are the standard experimental protocols for induction of lung fibrosis model? Do you have recommended concentrations, treatment durations, and cell or animal types for its application in fibrosis induction?
Bronchiolar Fibrosis Murine Model via Intratracheal TGF-β1 Instillation This represents a well-established modeling approach, as detailed in PMID: 12947136. The protocol involves intratracheal delivery of human TGF-β1 to induce peribronchial fibrosis in BALB/c mice, with validated consistency across multiple strains (ICR, A/J, C57/BL6).
Procedure:
Fibrosis Assessment:
- Histological Evaluation: Lung sections are stained with Sirius Red to quantify subepithelial collagen deposition.
- Biochemical Quantification: Hydroxyproline content (a surrogate for total collagen) is measured in microdissected airways, with significant increases detectable at ~4 weeks post-induction.
- Molecular Profiling: Elevated mRNA levels of type I and III collagen are observed in laser-captured microdissected airways by 1 week post-TGF-β1 instillation.
The answer to this question comes from MedChemExpress (MCE) Technical Support.
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