Hi! Yes, you can use different lipases depending on the lipid type and the positional specificity you're targeting. For example:

Candida antarctica lipase B, often immobilized as Novozym 435:

It has high selectivity for the sn-1 and sn-3 positions of triacylglycerols. After hydrolysis, the sn-2 monoacylglycerol can be isolated and analyzed to determine the fatty acid at sn-2.

Candida rugosa lipase:

Has less regioselectivity, but can still be useful depending on your substrate and reaction conditions. It tends to

Phospholipase A2 (PLA2):

If you are working with phospholipids, PLA2 hydrolyzes specifically at the sn-2 position, allowing direct identification of the fatty acid located there.

After hydrolysis, you extract the lipids, separate the fractions (MAGs, DAGs, FFAs), convert to FAMEs, and analyze by GC or GC-MS.

Thank you so much for your reply. Currently, I want to analyze the positional distribution of fatty acids at sn-1 and sn-2 positions of cyanobacteria. The protocol states that the lipase enzyme from Rhizopus oryzae is used.

The analysis results should show 2 bands on the TLC analysis (1 band is diacyl lipid, and another is sn-1 fatty acid). but I just get one band.

I am struggling to fix the problem. where is my mistake