17 July 2023 1 5K Report

Dear researchers, I am performing heart staining with TTC. after staining, the tissue becomes hard, rough and shrinks and i am unable to take photographs. How I can avoid it?

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In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View

How to find statistical data of EEG signals?

Hi all, I hope everyone is doing good. I am working on Machine Learning, I am working on EEG data for which I have to extract statistical features of the data. Using mne library I have extracted...

29 January 2021 6,686 1 View

Can I use conventional PCR master mix instead of hot star Taq polymerase in DNA methylation studies or in bisulphite converted DNA ?

I am trying to predict age from DNA methylation. I am using conventional PCR master mix, but I didn't get my PCR product. Is it due to Master Mix? Should I use HotStar Taq Master Mix ?

08 December 2020 8,776 4 View

Why PDMS doesn't crosslink sometimes????

I am trying to fabricate a thin film structure of PDMS on a glass slide. I diluted PDMS (0.5 gm) with 5 ml Hexane and add the hardener (0.05 gm) in it. Sonicated it for 5 min, spin-coated a thin...

04 October 2020 1,454 4 View

How to add information from newspapers to a research paper?

I'm currently working on a research paper related to literature and COVID-19 Pandemic. I've collected some information from online newspapers. Now I want to include them in my research paper. But...

18 September 2020 4,522 8 View

Can I use 24 hrs continuous lighting source for in vitro culture ?

Generally, we maintain 16h photo period in culture room for plant tissue culture. But continuous 24 hrs light is also beneficial for plant growth. So, for an experiment can I use 24 hr photo...

11 August 2020 7,006 15 View

Researchers/ organizations working on gender issues in Vancouver?

Hello everyone, Please reach out if you work on gender studies, gender psychology, social psychology of gender, or gender-based discrimination. You can reach me here or write to me at...

05 August 2020 8,313 7 View

What are the effects of COVID-19 on educational outcomes especially students without resources?

COVID-19 brought closure of educational institutions and lockdown. The teachers started online teaching. However, there are students that lack the required resources, internet and other devices....

04 August 2020 7,466 20 View

How to evaluate students' performance in online learning during COVID-19 lockdown?

Due to COVID-19 lockdown, the teachers are asked to take online classes. Besides imparting instruction, the teacher also has to conduct different types of assessments to evaluate students'...

23 July 2020 1,725 13 View

Data collection from ICRG index from 2009-2019 for political risk?

Hi, I am currently doing research on topic related to political risk for which I need political risk monthly data from ICRG Index. I need help for collection ... I need data for Asian countries......

13 July 2020 4,679 2 View

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Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

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Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

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25 February 2021 8,140 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

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