I am planning to stain a cell surface protein by immunofluorescence and then take readouts by plate reader. As I would like to detect cell surface protein, I guess cells should not be fixed. I have seen protocols for cells grown on coverslip but not directly in 96 well dish. Any kind of information/ protocol is welcome. Thank you.
Hi Ravindra Deshpande
Yes, you can perform ICC in 96-well plates.
Please find the General protocol attached.
Yes you can, and not just immunofluorescence. You can do immunoperoxidase staining and use bright field microscopy. It is better to fix the cells first, and permeabilize the cells if you need to detect intracellular proteins or intracellular domains of surface proteins.
May I add, you better use a glass bottom dish for ICC in plates, and if it's not lipid sort of things you want to detect, better to fix the cell and reduce detergent.
Hi Ravindra Deshpande , check this link for an easy protocol, its might be working for you.
https://biotium.com/tech-tips/protocol-immunofluorescence-staining-of-cells-for-microscopy/
- Badges
- Science method
- Similar topics
- Biological Science
- Histology
- Staining