I am new to creating solutions, and any help would be beneficial!
I am attempting to get yeast to secrete recombinant protein using the mating factor alpha pre-pro leader sequence. However, the yeast that I am working with is of the opposite mating type (matA)....
18 June 2023 6,572 3 View
Hello, Does anyone have any advice on how to measure soil moisture or hydraulic conductivity in a microbial greenhouse experiment? Seedlings will be grown in D40 cones and half of the sample will...
27 September 2021 2,301 3 View
Long story. I can't use any normal endogenous control in for my westerns, so I decided to go with a whole protein stain with amido black. The amido black solution I purchased has isopropanol in it...
19 August 2021 7,523 5 View
We are currently heating 20 mL 95-98% H2SO4, 2 Kjeldahl tabs (each are 1.5 g K2SO4 and 7.5 mg Se), and 1.0 gm protein sample (fish). We have continued the heating process to upwards of 4 hours....
21 December 2020 5,694 3 View
I aim to do a PhD in the future (on the general topic of ecology and biogeography) and would like to do a masters before this to make myself a more competitive candidate. However, I am pretty...
03 October 2020 6,924 2 View
Here are the steps I'm taking: - ABTS solution: 192 mg of ABTS in distilled water to 50 mL. Store this solution in an amber flask. - Potassium persulfate (140 mM): Mix 378.4 mg of the salt with...
24 September 2020 5,309 5 View
Hello, all! We are using the Kjeldahl method to determine nitrogen content of fish samples. We do not have an automatic Kjeldahl analyzer, so we are using basic glassware. Regarding the second...
23 August 2020 236 6 View
We are testing various physical parameters of both fish and tomato samples, such as acidity, color, texture, fat content, etc. What is an appropriate sample size for these types of tests?...
18 August 2020 3,826 3 View
My reference paper says to mix ABTS solution (mine is already prepared) with a potassium persulfate (2.45 mM) solution. Can anyone provide instructions on how to prepare the potassium persulfate...
07 January 2020 198 9 View
Hello everyone! I regularly use the Sigma BrdU cell proliferation kit for my cell assays, and due to the nature of the kit I always run out of some reagents before others. This means I keep...
09 May 2019 1,696 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View
I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...
15 July 2024 9,827 1 View
I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...
11 July 2024 4,832 0 View
I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...
24 June 2024 9,319 0 View
I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...
12 June 2024 6,087 0 View
I have seeded HeLa cells in a calculation of 10000 cells per well in a 96 well plate. Then I went for treatment in the next day. Then at last, I added 7ul of 5mg/ml solution of MTT reagent into...
12 June 2024 1,460 3 View
Is it possible to do FACS on snap frozen cells ? They were isolated and directly dropped in liquid N2. They were not frozen with DMSO or FBS.
11 June 2024 8,915 0 View
Hello, I discovered that a compound that I use, which get integrated into RNA, might have an unspecific impact on mRNA stability of a gene of interest. To confirm that, I pretreated my cells for...
10 June 2024 276 2 View