How to complete digestion step of Kjeldahl method?

We are currently heating 20 mL 95-98% H2SO4, 2 Kjeldahl tabs (each are 1.5 g K2SO4 and 7.5 mg Se), and 1.0 gm protein sample (fish). We have continued the heating process to upwards of 4 hours....

21 December 2020 5,480 3 View

Is an MRes or a skills based MSc better for moving onto a PhD?

I aim to do a PhD in the future (on the general topic of ecology and biogeography) and would like to do a masters before this to make myself a more competitive candidate. However, I am pretty...

03 October 2020 6,680 2 View

What is an appropriate sample size for physical tests?

We are testing various physical parameters of both fish and tomato samples, such as acidity, color, texture, fat content, etc. What is an appropriate sample size for these types of tests?...

18 August 2020 3,464 3 View

Can I replace Sigmas BrdU ELISA kit components with DIY reagents?

Hello everyone! I regularly use the Sigma BrdU cell proliferation kit for my cell assays, and due to the nature of the kit I always run out of some reagents before others. This means I keep...

09 May 2019 1,512 3 View

Trying to run RT-PCR for PDCD4 gene in human serum, can't get any values. Checked primers and they seem okay, any suggestions?

We are having difficulty detecting PDCD4 in serum. We have ordered an ELISA to attempt this, but have not tried it yet. Afraid something is wrong with serum.

08 January 2018 4,637 3 View

Anyone culturing CTLL-2's? Cell density tips?

11 February 2016 8,501 2 View

How to quantify protein concentration......?

I have carried out some nanoparticle treatments on a set of cells, which I plan to use for western blot. Of 7 treatments, one set of cells has turned red, while another dark purple. I think this...

18 January 2016 5,512 7 View

Is anyone using Abcam Anti-CD40 ab13545 for Western blotting?

I have just begun western blots looking at CD40 in immune cell pull downs. The antibody daasheet states the band will be apparent at 41kDa, although the predicted size is 34kDa. I am not too...

24 June 2015 9,594 2 View

Is it possible to use the same primary antibody from different species on the same blot and then use the different secondaries to visualize?

I am using the LiCor Odyssey to visualize my blots. I am trying to decide which primary antibody is the best to use for future experiments. One primary is mouse and the other is rabbit, I have 2...

21 January 2015 9,938 10 View

Series of Tau antibodies for Western blot analysis in human tissue that will span the entire tau protein?

I am looking to find a series of commercially available antibodies for western blot analysis on human tissue covering the expanse of the tau protein (i.e. antibodies detecting the 6 isoforms- both...

27 October 2013 4,469 2 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

How can I make sure that BCTC doesn't fall out of solution/form DMSO crystals when treating cells?

I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...

14 February 2021 5,633 1 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

DMSO concentration in cell culture? Precipitating while PBS is added?

I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it...

11 February 2021 1,136 3 View

What are the main conditions suited for synthesizing a PVDF thin film by solvent casting method?

I have been trying to synthesize PVDF thin film by solvent casting method. I started by using 2:3 DMSO: acetone as solvents. The solution was stirred for 3hrs at 60 deg and was dried at 90 degrees...

09 February 2021 8,850 3 View

H2O2 scavenging activity problem??

When I do the test of plant against the free radicals of H2O2 the control had a value about 0.9126 The critical point is when I prepared the extract with DMSO the absorbance appear more than 2...

09 February 2021 6,430 2 View