Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

How can I add the metal oxide NP's to the cell lines? I have to make some drug and NP conjuhgates. How much concentration to use? Need some help?

Hi everyone. I am working on metal oxides. I need to make some conjugates of drugs and NP. But I am unsure of what concentrations to make. I have read some articles, but they haven't mentioned...

10 February 2021 3,372 2 View

Can I use a time series dependent variable in a panel regression?

Hi all Can I run a Gmm model with a time series dependent variable? I have a sample of 12 countries and years from 2000 to 2018

03 February 2021 6,491 6 View

the drug loaded nanoparticles?

Hi, I am new to this cancer cell lines field. My research is using the drug loaded nanoparticles and their effects on cancer cell lines. have a question regarding the drug loading. I have...

24 January 2021 8,258 3 View

How to calculate an endotoxin limit with a drug concentration in vp/mL?

Hello, I am trying to validate an endotoxin method for an excipient. I need to calculate my endotoxin limit. My excipient is 0.1% final product and the drug concentration is expressed in vp/mL....

20 January 2021 1,262 1 View

Is it possible to determine nitrogen concentration colorimetrically, using spectrophotometer in macroalgal tissue?

I'm trying to estimate total nitrogen content in seaweeds without using the Kjeldahl method. Thanks in advance

20 December 2020 9,190 2 View

Why is my passive reference dye (ROX) decreasing in fluorescence as my target (FAM) increases during qPCR?

I am running qPCR on eDNA samples from rivers and have found that the vast majority of my samples show a decrease in fluorescence in my passive reference dye (ROX) while my target dye (FAM)...

15 December 2020 723 4 View

Is it possible to trap air and convert it to plasma?

How can I trap air and then convert the trapped air into plasma?

06 December 2020 624 7 View

How to deal with missing data for Reliability Analysis?

Hi, I have a dataset with missing values. I want to do a reliability analysis with SPSS and then implement the data in Mplus. Mplus can handle missing data due to the FIML. I am unsure how to...

21 November 2020 4,658 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View