I am currently in need of an antibody targeting gene X, specifically focusing on a distinct splice variant where the major variance lies within the N-terminal region. Unfortunately, suppliers like Cell Signaling and Abcam don't provide epitope information, which is crucial for ensuring specificity towards my variant of interest.
Does anyone have suggestions or experiences on how to acquire epitope information from antibodies offered by these suppliers? Alternatively, could anyone recommend reliable strategies or resources to verify the specificity of an antibody towards a particular N-terminal epitope of a specific splice variant?
Your insights or guidance on obtaining this essential information would greatly aid my research. Thank you in advance for your help!
Hello, couple of options.
1. If you have checked the antibody datasheet and the information is lacking, check the literature that has used the antibody or message the supplier for technical support. Abcam has a trial program that can allow you to test their products on a system where it was not previously validated and upon sharing your review, you can get another product with the same cost free of charge
2. You can use Antibodypedia (https://www.antibodypedia.com/), a platform for sharing antibody validation data.
3. In the worst-case scenario, having good positive and negative controls can be helpful.
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