Which method is more efficient for producing multi-copy strain in Pichia pastoris, In-vitro multimerization or PTVA?

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Issues in coating of cathode material on Al foil, why we are not getting proper coating and results when the cathode was coated on Al-foil..?

We are trying to coat some cathode materials on Al-foil ( ex - LiFePO4 , S@CNT, etc). When we coated the Al foil with the cathode (ex - S@CNT) material , the cycling result was annoying. But the...

09 July 2024 5,123 4 View

How does AR integration in online luxury retail impact UK consumer purchases, and how can engineering management optimize this?

This concise research question focuses on two main aspects: Impact of Augmented Reality (AR) on Purchasing Decisions: The question investigates how the use of AR technologies in online luxury...

17 May 2024 1,859 2 View

How to solve this KKT condition. Plzz provide me full answer?

This is formulation given in "Competitive resource allocation in an attacker-defender game: When citizens become targets of being ingratiated" paper. But the solution approach is not provided.

17 March 2024 5,902 0 View

How to couple two phase flow phase field and heat transfer module in comsol multiphysics?

Hello, I am trying to simulate two phase flow (laminar and phase field module of comsol) inside a pipe with the heat transfer from the pipe wall. when I run the model without heat transfer...

25 December 2023 6,968 2 View

Which module in comsol multiphysics can be used for microstructure modelling?

Hello all, We are trying to simulate microstructure formation during the die casting process using comsol multiphysics software. However, we stuck in applying various governing equations in...

01 December 2023 3,843 0 View

How to do cluster analysis using HYSPLIT model?

I am trying to calculate the backward trajectories for bioaerosol samples. How to classify the trajectories through a clustering analysis? using NOAA HYSPLIT trajectory model. I would greatly...

27 April 2023 3,962 2 View

How to convert micro-fibrous structure into nano-fibrous structure?

Is there any method to convert micro structure of material into nanofiber? I would greatly appreciate any help you could give me with this.

17 April 2023 5,030 2 View

How to run positive matrix factorization (PMF) for source apportionment of PM particles?

Dear all, I am using EPA-PMF (Version 5) software for source apportionment of PM-bound heavy metals. I would greatly appreciate any help you could give me with this. Thank you

14 April 2023 639 2 View

What is the role of airborne bioaerosols in Oxidative potential of atmospheric particle?

The OP of bioaerosols can be assessed through the cell-free DTT assay. How do bioaerosols induce reactive oxygen species (ROS) generation? I would greatly appreciate any help you could give me...

12 April 2023 310 2 View

Procedure for oxidative potential of airborne Bioaerosols?

DTT assay procedure for OP of bioaerosols?

09 April 2023 4,066 2 View

How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

I need help with cloning a 200 bp insert and a 4,7 Kb pEGFP-N1 vector. How can I do it?

I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...

09 July 2024 4,277 1 View

Cloning restrction digestion needed before sequencing?

Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...

04 July 2024 7,977 2 View

Why I am unable to get the expected insert at the cloning site?

I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...

02 July 2024 5,448 2 View

What could be the expression profile of alkaline phosphatase from lactic acid bacteria?

I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...

02 July 2024 5,332 3 View

List Plasmid suitable for Homology cassette in CRISPR-Cas9 protocol for Knock-In in mammalian cell line ?

Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...

25 June 2024 1,197 0 View

How can I convert remote sensing (RS) images to vector data automatically?

I am working on a project that involves analyzing remote sensing (RS) images. I need to convert these images into vector data for further analysis. I am looking for automated methods or software...

22 June 2024 6,165 2 View

Asking for a vector, containing Hygromycin B resistance gene?

In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...

19 June 2024 7,822 3 View