Is there a way to identify if a mutation in a promoter is increasing gene transcription? any application we could run a test for different mutations?

11 December 2023 1 5K Report

Michael J. Benedik

This has been done experimentally for decades, you can either use a reporter gene to measure level of expression with different promoter mutants, or you could use an in vitro technique like RT-PCR to measure the amount of transcript produced.

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