Message ID#68 Error: Error occurred while trying to set up a Blast Object from CGI context: CFastaReader: Near line 1, there's a line that doesn't look like plausible data, but it's not marked as defline or comment.
I think you have a problem with the format of your sequence(s). Try again using fasta format. Fasta format is as follows:
>NAME-OF-SEQUENCE
CTGCGTAGTCAGTACCAAGGTTGAGGACCTTAGGACCCTA...
>NAME-OF-SEQUENCE-2
CCCCCTGCGTAGTCAGTACCAAGGTTGAGGACCTTAGGA...
for each sequence you want to blast, you need to add the sequence name (no spaces) preced by the symbol ">". Then, in a new line, add your sequence (nucleotide or protein) with no spaces in a single line. Then new line with the next sequence name (do not forget the >)...
This needs to be in plain text (TXT format). In windows use notepad to prepare the sequnces, in mac use TextEdit (but change it from rich text file to plain text).
Good luck!
Dear Alfonso,
I've done all those. I think the problem is from the large number of query sequences.
Hi Charles,
That problem is very easy to test: submit only the first 2 sequences. If you are get a result then you need to split your sequences in groups (first half and second half for example).
Good luck!
PS: I am assuming you used blastn (http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome) or blastp (http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastp&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome) suites at ncbi. I just saw they updated blast very recently.
Splitting my sequences might help but I think it'll be too cumbersome for me to analyse the results. Well I tried the stand alone program from NCBI and I keep getting this error message:
NCBI C++ Exception:
TO "..\..\..\..\..\..\src\objtools\readers\fasta.cpp". line 2167: Error: ncbi::objects::CFastaReader: : PostWarning - CFastaReader: Near line 1, there's a line that doesn't look like plausible data, but it's not marked as defline or comment. .
I'll be grateful if anyone can help me with solution to this problem.
what is the first character of your file? have you tried the on line version?
good luck!
Dear Alfonso, splitting the sequences worked. I did the blasting with the online version. Thanks for your answers.
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