How to reduce noise in sequencing reaction when dealing with mitochondrial genome?

06 June 2016 1 5K Report

I have amplified the whole mitochondrial genome in 9 fragments and use 62 set of overlapping internal primers for sequencing. I face problems while sequencing. Each fragment is further divided into smaller fragments for the same template that i use i get different sequencing results. Can anyone help me please?

Brian Thomas Foley

I think you need to be more specific about the problem if you want a good answer. Can you upload some sample of the results maybe?

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