I want to stain my bacterial cells to highlight the nucleus on the surface where my protein is attached. I have tried using Hoechst, but the signal in flow cytometry was too weak, resulting in a low cell gate. Is there a better dye or an alternative technique to achieve this visualization?
Anton Lennikov
Bacterial cells do not have a nucleus; therefore, Hoechst or DAPI is less localized and produces weaker signals than eucaryotic cells. I would recommend SYTO9 for your flow experiment counterstaining. If you want to label the bacterial cell membrane for high resolution/high magnification confocal microscopy, fluorescent Wheat germ agglutinin (WGA), lectin conjugate will allow you to reliably visualize the cell wall of gram-positive bacteria.
1 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- RNA
More Cássio Prinholato da Silva's questions See All
Similar questions and discussions