Contact experts in Real-Time PCR to get answers
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Questions related to Real-Time PCR
After the insect feeds, do you have to threat the medium in any way (use an extraction protocol)? can you analyse it directly (using the medium as template)? I'll be using a real-time PCR protocol...
30 August 2017 8,203 1 View
I would like to determine gene relative expression in real-time PCR using the comparative CT method (2-ΔΔCt), using three internal control instead of one gene.
08 August 2017 2,981 7 View
We are about to start our diagnostic lab for Fragile X, and the real-time machine that appeals to us is LineGene 9600 Plus...
08 August 2017 3,075 4 View
08 August 2017 4,914 1 View
02 August 2017 467 2 View
Our lab just obtained some 5XFAD Het mice that we plan to use. Our plan is to use WT, Hets, and Homozygous animals and was wondering if anyone had a better way to genotype them besides using...
07 July 2017 9,460 4 View
Your toughest technical questions will likely get answered within 48 hours on ResearchGate, the professional network for scientists.
07 July 2017 6,560 0 View
We have performed the anti-proliferation assay and nuclear receptor binding assay and got an IC50 value for the target compounds. I am planning to perform an RT-PCR experiment for analyzing the...
06 June 2017 6,875 2 View
When running a 384 well plate on the CFX 384, I won't necessarily be filling every single well with some kind of sample or control. Do I need to fill the empty wells with water or something or can...
06 June 2017 739 3 View
I optimized pcr by touchdown pcr and I got one specific sharp band on agarose gel, but now I want to do real-time pcr and there isn't any option in software to select touchdown pcr. so I have to...
06 June 2017 1,315 3 View
I have personally experience that its relatively tough to publish in a good journal in social science, for example let us suppose in the field of finance, marketing and so on. In comparison i have...
04 June 2017 7,397 10 View
I have a question to Jochen Wilhelm and other experts in the field. I am a physician scientist currently working in basic science. We work on rodent experimental models and use qPCR a lot. We...
05 May 2017 8,794 7 View
I want to test a specific promoter mutation to see if it would transcribe and translate. What method (protocol) would be more convenient to carry this out? I tend to think Luciferase would be better
05 May 2017 1,617 3 View
HEPG2 cells (human hepatocellular liver carcinoma cell line) were incubated for 72 hours with different conditions:1. Medium containing cholesterol (= control group)2. Medium without cholesterol3....
05 May 2017 3,123 1 View
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05 May 2017 722 1 View
I performed real-time PCR to find out the expression of different genes in osteoarthritis and normal rat model and for that, I used GAPDH as a standard. The issue I am facing is that the GAPDH...
04 April 2017 6,384 2 View
Hi, I am doing a relative quantification of genes (A and B) from same family.I am using Actin as calibrator. I have primers specific for gene A and primers specific for gene B. If I know these two...
04 April 2017 418 4 View
Dear Colleagues. I am working on c2c12 cell line. The cell line was transfected and stabled. Stable cells exposed to hypobaric oxygen champer (with hypoxic buffer) for 90 minutes and normal media,...
04 April 2017 3,929 0 View
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04 April 2017 6,412 0 View
Usually, quantal size and release probability were determined from the amplitude and frequency miniature EPSCs(mEPSC), I wonder why does NMDAR-mediated EPSCs by MK-801 can be used to examine basal...
30 March 2017 943 5 View
I'm planning to produce a cheese with the addition of Lactobacillus casei NRRL B-441, and investigate the survival of the strain during the ripening period. Since there might be...
03 March 2017 5,397 3 View
i am to real-time PCR methods and i am trying to quantify gene copies of viruses from environmental samples. I have run Real-Time PCR with the Standard Curve method and then i decide to run the...
03 March 2017 9,975 6 View
hello every body i want to design primer for my sequences but because of cross-pollination nature of my plants (Thymus sp) my sequences are heterozygot (they have SNP). I tried to design primer...
16 February 2017 3,724 3 View
When performing conventional RT-PCR we did not detect TP53 expression, but when performing real time PCR with a Taqman assay for the exon 10-11 boundary, we detected expression. Maybe there´s a...
03 February 2017 7,541 1 View