Contact experts in Real-Time PCR to get answers
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Questions related to Real-Time PCR
I want to test a specific promoter mutation to see if it would transcribe and translate. What method (protocol) would be more convenient to carry this out? I tend to think Luciferase would be better
05 May 2017 1,606 3 View
HEPG2 cells (human hepatocellular liver carcinoma cell line) were incubated for 72 hours with different conditions:1. Medium containing cholesterol (= control group)2. Medium without cholesterol3....
05 May 2017 3,115 1 View
I?
05 May 2017 715 1 View
I performed real-time PCR to find out the expression of different genes in osteoarthritis and normal rat model and for that, I used GAPDH as a standard. The issue I am facing is that the GAPDH...
04 April 2017 6,367 2 View
Hi, I am doing a relative quantification of genes (A and B) from same family.I am using Actin as calibrator. I have primers specific for gene A and primers specific for gene B. If I know these two...
04 April 2017 409 4 View
Dear Colleagues. I am working on c2c12 cell line. The cell line was transfected and stabled. Stable cells exposed to hypobaric oxygen champer (with hypoxic buffer) for 90 minutes and normal media,...
04 April 2017 3,910 0 View
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04 April 2017 6,403 0 View
I'm planning to produce a cheese with the addition of Lactobacillus casei NRRL B-441, and investigate the survival of the strain during the ripening period. Since there might be...
03 March 2017 5,389 3 View
i am to real-time PCR methods and i am trying to quantify gene copies of viruses from environmental samples. I have run Real-Time PCR with the Standard Curve method and then i decide to run the...
03 March 2017 9,963 6 View
hello every body i want to design primer for my sequences but because of cross-pollination nature of my plants (Thymus sp) my sequences are heterozygot (they have SNP). I tried to design primer...
16 February 2017 3,706 3 View
When performing conventional RT-PCR we did not detect TP53 expression, but when performing real time PCR with a Taqman assay for the exon 10-11 boundary, we detected expression. Maybe there´s a...
03 February 2017 7,517 1 View
02 February 2017 9,799 4 View
In the case I do not have a standard curve.
02 February 2017 574 0 View
I am trying to figure out which C4A are long and which are short (and the same for C4B). We have a plan to do a study of complement component 4 using RT-PCR on the Biomark HD. We have decided...
02 February 2017 1,043 0 View
02 February 2017 7,350 1 View
Do you think the most accurate method is real-time pcr?
24 January 2017 5,414 7 View
This will be long, but I am out of ideas and require help. I am having difficulty with my real time PCR as I am obtaining multiple products in each sample. I am looking for expression of JDP2 in...
17 January 2017 9,887 6 View
I'm going to expose cells to hypoxic conditions, in order to establish the function of a synthetic promoter containing HRE's. For control sake, I want to see if cells actually developed hypoxic...
11 January 2017 6,574 3 View
Hi, I am working on circulating RNAs available in human plasma. After cDNA synthesis using TaKaRa Kit I performed qPCR (with amplicon) with GAPDH and U6 primers, but the result is a bit confusing...
02 January 2017 7,087 4 View
01 January 2017 6,612 5 View
To check the primer specificity we do run a melt curve analysis and a single curve implies the formation of a single product. But I have seen in many papers people reconfirming the specificity by...
01 January 2017 3,305 10 View
Hello I am working on rat models of acute kidney injury that induce with glycerol and ischemia reperfusion. I want to check therapeutic effects of mesenchymal stem cells of T cells subset in...
01 January 2017 5,193 1 View
Hi, I'm using the rotor gene q for my CPM assay and in the setup of the analysis I have to adjust the ramping rate to be 2˚C/min but the only way to do this is to adjust (wait for -- seconds for...
01 January 2017 8,788 0 View
01 January 2017 9,921 8 View