Contact experts in Real-Time PCR to get answers
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Questions related to Real-Time PCR
Hi every body, I want to set up real time PCR for one gene, but after doing it I get two or three peaks in melting curve of real time PCR. The designed primers for my gene is very specific. After...
04 January 2018 1,427 13 View
Hi, i want to analyze the expression of SNAIL 1 and 2 in various cancer cell lines by real-time PCR. Is it always necessary to perform a housekeeping gene validation with geNorm according to...
01 January 2018 7,357 3 View
I am working with highly mutagenized Bacilli species and wish to measure expression level of my favorite genes by real-time PCR. As I know, there are no reliable internal reference genes for...
01 January 2018 9,971 1 View
Does anyone have any articles on detection of SEN virus, using Real-time PCR Techniques?
01 January 2018 3,915 2 View
i am work with antibiotic resistance genes in wastewater with Real Time PCR analysis, like Tetm gene, Sul1 gene, Intl1 gene. who can help in some questions ? thank you Ammar
01 January 2018 7,543 2 View
I synthesized an existing probe in the literature (FAM-MGB). The probe is marked with the quencher MGBEQ (eclipse) (Company Eurofins Genomics). However, the probe did not amplify anything, it may...
12 December 2017 2,925 0 View
Hi, has anyone exprience, the luciferase assay resulted in increasing the expression but by the analysis using real-time it is low/no expression at all. Is it because post-transcriptional...
11 November 2017 2,170 4 View
Dear there is a plasmid which has two nucleotide sequences from two genotypes ( C2 and A types ) We have to prove each of sequences are expressed well not only one sequence As you know, the...
10 October 2017 2,876 4 View
Hi everyone, I am a bit curious about the protocol of collecting the mouse liver for real-time PCR. As I would like to check the expression of the gene under two different conditions. However,...
10 October 2017 8,302 0 View
I am trying to operate a real-time PCR. How can I find a repetitive element on the genome of a specific organism?
09 September 2017 7,829 2 View
Hello, I use a commercially available real-time PCR kit for poultry virus detection. I have used this kit for some preliminary experiments but need the primer sequences for further research. Is...
09 September 2017 5,187 3 View
I want to compare the Traditional CFU count of bacteria with Real-time PCR method, but I m having some issues with the making standard curve. So please if anyone is willing to help I will really...
09 September 2017 3,915 3 View
04 September 2017 1,623 3 View
After the insect feeds, do you have to threat the medium in any way (use an extraction protocol)? can you analyse it directly (using the medium as template)? I'll be using a real-time PCR protocol...
30 August 2017 8,183 1 View
I would like to determine gene relative expression in real-time PCR using the comparative CT method (2-ΔΔCt), using three internal control instead of one gene.
08 August 2017 2,965 7 View
We are about to start our diagnostic lab for Fragile X, and the real-time machine that appeals to us is LineGene 9600 Plus...
08 August 2017 3,068 4 View
08 August 2017 4,905 1 View
02 August 2017 459 2 View
Our lab just obtained some 5XFAD Het mice that we plan to use. Our plan is to use WT, Hets, and Homozygous animals and was wondering if anyone had a better way to genotype them besides using...
07 July 2017 9,454 4 View
Your toughest technical questions will likely get answered within 48 hours on ResearchGate, the professional network for scientists.
07 July 2017 6,549 0 View
We have performed the anti-proliferation assay and nuclear receptor binding assay and got an IC50 value for the target compounds. I am planning to perform an RT-PCR experiment for analyzing the...
06 June 2017 6,868 2 View
When running a 384 well plate on the CFX 384, I won't necessarily be filling every single well with some kind of sample or control. Do I need to fill the empty wells with water or something or can...
06 June 2017 724 3 View
I optimized pcr by touchdown pcr and I got one specific sharp band on agarose gel, but now I want to do real-time pcr and there isn't any option in software to select touchdown pcr. so I have to...
06 June 2017 1,276 3 View
I have a question to Jochen Wilhelm and other experts in the field. I am a physician scientist currently working in basic science. We work on rodent experimental models and use qPCR a lot. We...
05 May 2017 8,778 7 View