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Questions related to Real-Time PCR
In the real-time experiment usually the melting peak is about 85 degree celcius. But I found for one of my gene of interest higher-Tm peak about 94 degree celcius. Has anyone observed this...
07 July 2018 6,452 2 View
Here I have met a problem: I ran my qPCR with "SYBR green master mix" with actin primer to quantify my data relatively in use of "delta delta Ct." The data shown here is strange, because there are...
07 July 2018 7,677 3 View
i do a study for a gene expression in HCC patients compared to healthy person using real time pcr. i have analyzed ct for gene of interest and house keeping gene in each sample either HCC or...
07 July 2018 7,171 13 View
The viral RNA obtained from a patient with influenza symptoms was analyzed and the results for a real-time PCR were negative for the influenza A virus and positive for the AH1NI virus. The...
07 July 2018 3,887 4 View
Goal: Quantification of a RNA-Virus in several tissues of bank voles Wanted: housekeeping genes
07 July 2018 8,303 1 View
Hello! I am trying to run real-time pcr but whenever the run ends it shows me an error massege " Analysis cannot proceed: not enough samples defined". However I still can get an exponential curve...
07 July 2018 7,504 0 View
Hello Everyone, I have obtained a number of transgenic potato plants via overexpression of one of the genes from defensive pathways to enhance the resistance against the viruses. Then I evaluated...
07 July 2018 3,212 8 View
06 July 2018 9,220 3 View
Hi. I'm doing qPCR on healthy viroid sample. I got N/A or zero value during my amplification but some of my friends got cq value for healthy samples. It make me worried. Healthy sample suppose...
09 June 2018 2,649 3 View
I have qRT-PCR data of 4 transgenic line and 1 non-transgenic line. Non-trangenic line has GOI Ct value zero and HKG has Ct value because non-transgenic has no transgene/GOI, that's why no Ct...
06 June 2018 7,851 4 View
06 June 2018 3,664 3 View
Dear colleagues, I work in a private company, which is serving labs. One of our customer has a serious problem with contamination in the real-time pcr. First contamination was detected in February...
06 June 2018 9,416 3 View
for us is very importan the purifiaction and extration of the mRNA in tissue previusly fixed with paraformaldheide, thats possible? or may be we can used other solutions for preserve the sample,...
05 May 2018 9,148 0 View
I have been studied the effect of tamoxifen and silver Nanoparticles on HepG2 cell line. I have been made 3 groups tamoxifen only, silver nanoparticles only, and a mixture of tamoxifen and silver...
05 May 2018 2,057 9 View
I would like to change the 36 well rotor for the 72 well rotor, can anyone tell me how to do it?, this is not explained in the manual.
05 May 2018 3,700 3 View
I have been trying to perform absolute qPCR with a number of genes performing nitrogen transformations in prokaryotes. I've had trouble getting good efficiency (>90%), even after experimenting...
05 May 2018 9,280 1 View
I am designing a one-tube nested real-time PCR. Only one probe will be used and the signal will be only collected from the second stage. Any suggestions for primer and probe TM design?
02 May 2018 3,021 4 View
Has anyone used q-PCR for cell counting? How is its accuracy compared to colony formation assay and other methods? Theoretically, I can insert unique genetic markers/barcodes in the target genomes...
24 April 2018 1,734 4 View
Is there any one that interest to cooperate and find fund for below project? Evaluation of Gene Expression Pattern in Chenopodium quinoa under heat stress by Real-time PCR Proposal of these...
04 April 2018 7,051 0 View
04 April 2018 9,698 5 View
In a project we need 20 ml of blood, do we take this sample at a single time or twice (for instance 2 consecutive day)? What do we do with blood donation? Probably our patients are not willing to...
04 April 2018 7,007 4 View
The title's paper is: Development and validation of a rapid and reliable method for TPMT genotyping using real-time PCR. Author: Lorenz et al.2012. Clin Lab. 58(9-10):959-71 Thanks everyone!
04 April 2018 1,064 1 View
I want to evaluate some miRNAs in exosomes by Real-Time PCR., and I don't know what would be the best control to normalize. I added the Cel-miR 39 as an exogenous control in all my samples, but...
04 April 2018 1,374 0 View
I want to study the quantitative gene expression by real time PCR. For this i need to isolate total RNA and then do cDNA synthesis. I'm little confused that we should harvest the bacterial cells...
03 March 2018 1,510 6 View