Contact experts in Real-Time PCR to get answers
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Questions related to Real-Time PCR
Our study comprises of PCR analysis of DNA isolated from fecal samples at two time points (at the baseline and after intervention). We have carried out PCR and have obtained the Ct values....
11 November 2018 801 8 View
Hi everybody, I am developing a real-time PCR based animal MHC typing assay. I need to quickly decide whether SYBR Green or TaqMan chemistry should be used. I am focusing on SYBR Green now...
11 November 2018 5,927 5 View
Hi! I would like to know if anyone has the protocol for HPRT (housekeeping gene) conventional PCR (cyles, temperature...)? I've been searching on internet but I just found for real-time PCR. Can...
23 October 2018 8,059 2 View
10 October 2018 9,637 2 View
Hello colleagues, This question has been intriguing me and I have not found a valid answer for it in the literature. I know that exosomes do not have a specific loading control/housekeeping gene,...
10 October 2018 9,434 4 View
Hi, I am currently working on my realtime pcr data. I had analysed my result, but there is something that confused me. Is it possible to have a big fold change (5-fold change) but it is...
10 October 2018 5,954 7 View
Hello everyone! In your experience, changes in the expression of which genes better represent synaptic growth and activity? I look for a marker of vesicular pool (quantity of synaptic vesicles,...
10 October 2018 7,923 4 View
Hi, I have assessed 4 SNPs by TaqMan genotyping assays from Applied Biosystems using StepOne plus realtime PCR system. Now, I tend to analyze my results via TaqMan genotyper software V 1.0.1....
18 September 2018 9,778 5 View
I am working with qPCR on Lactobacillus plantarum WCFS1 and there is a high expression level of 16S rRNA gene comparing to the other genes. Therefore, I am looking for the other housekeeping genes...
09 September 2018 8,564 3 View
09 September 2018 6,982 6 View
Hi I would like to know if mycoplasm infection in cell cuture can alter the real time PCR. tks
09 September 2018 333 0 View
Hi everyone, I tried many times to transfect my fibroblast with my plasmid, but so far, it never worked. I tried to vary different factors such as 1) Day of treatment (24 to 72 hours) 2)...
09 September 2018 2,713 0 View
I have recently ordered the Taqman RNA-to-CT One-step kit in order to assess the expression regulation of 12 genes involved in the one-carbon metabolism and oxidative stress. Before starting with...
09 September 2018 9,632 3 View
Dear All, Does anyone share the experience about the measurement of IGF-1 in cells? I tried several times for measuring the level of IGF in cells by using the cell lysate or medium. However, I...
09 September 2018 7,442 3 View
06 September 2018 5,669 3 View
My cDNA synthesis kit said that the maximum amount of RNA should be used in cDNA synthesis is 5μg, so I want to know if I use more than this amount and do Real-Time PCR after that, will it cause...
14 August 2018 1,114 4 View
Sorry for the poor image quality, I am working with a fairly old real-time PCR system (Bio-Rad MyIQ on iQ 5 version 2.0 software). I am using primers, cDNA, and supermix (Bio-Rad iQ SYBR Green)...
13 August 2018 3,803 4 View
Hi all, Can anybody suggest me the steps required to perform quantitative real-time PCR analysis of mRNA expression data. Actually, I have some differentially expressed genes and I want to...
08 August 2018 8,371 0 View
Hello, every genius I am looking for a protocol to extract adenoviral DNA. Is it fine to extract it by using a QIAamp DNA Mini Kit (ID=51304)? The technical supporting response to my question is...
08 August 2018 3,640 0 View
08 August 2018 2,215 5 View
08 August 2018 8,888 3 View
In the real-time experiment usually the melting peak is about 85 degree celcius. But I found for one of my gene of interest higher-Tm peak about 94 degree celcius. Has anyone observed this...
24 July 2018 8,096 2 View
Here I have met a problem: I ran my qPCR with "SYBR green master mix" with actin primer to quantify my data relatively in use of "delta delta Ct." The data shown here is strange, because there are...
23 July 2018 9,722 3 View
07 July 2018 6,344 2 View