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Questions related to Real-Time PCR
Hi everyone, I tried many times to transfect my fibroblast with my plasmid, but so far, it never worked. I tried to vary different factors such as 1) Day of treatment (24 to 72 hours) 2)...
09 September 2018 2,705 0 View
I have recently ordered the Taqman RNA-to-CT One-step kit in order to assess the expression regulation of 12 genes involved in the one-carbon metabolism and oxidative stress. Before starting with...
09 September 2018 9,624 3 View
Dear All, Does anyone share the experience about the measurement of IGF-1 in cells? I tried several times for measuring the level of IGF in cells by using the cell lysate or medium. However, I...
09 September 2018 7,422 3 View
06 September 2018 5,660 3 View
My cDNA synthesis kit said that the maximum amount of RNA should be used in cDNA synthesis is 5μg, so I want to know if I use more than this amount and do Real-Time PCR after that, will it cause...
14 August 2018 1,100 4 View
Sorry for the poor image quality, I am working with a fairly old real-time PCR system (Bio-Rad MyIQ on iQ 5 version 2.0 software). I am using primers, cDNA, and supermix (Bio-Rad iQ SYBR Green)...
13 August 2018 3,772 4 View
Hi all, Can anybody suggest me the steps required to perform quantitative real-time PCR analysis of mRNA expression data. Actually, I have some differentially expressed genes and I want to...
08 August 2018 8,364 0 View
Hello, every genius I am looking for a protocol to extract adenoviral DNA. Is it fine to extract it by using a QIAamp DNA Mini Kit (ID=51304)? The technical supporting response to my question is...
08 August 2018 3,628 0 View
08 August 2018 2,202 5 View
08 August 2018 8,865 3 View
In the real-time experiment usually the melting peak is about 85 degree celcius. But I found for one of my gene of interest higher-Tm peak about 94 degree celcius. Has anyone observed this...
24 July 2018 8,082 2 View
Here I have met a problem: I ran my qPCR with "SYBR green master mix" with actin primer to quantify my data relatively in use of "delta delta Ct." The data shown here is strange, because there are...
23 July 2018 9,706 3 View
07 July 2018 6,334 2 View
07 July 2018 7,515 3 View
i do a study for a gene expression in HCC patients compared to healthy person using real time pcr. i have analyzed ct for gene of interest and house keeping gene in each sample either HCC or...
07 July 2018 6,913 13 View
The viral RNA obtained from a patient with influenza symptoms was analyzed and the results for a real-time PCR were negative for the influenza A virus and positive for the AH1NI virus. The...
07 July 2018 3,778 4 View
Goal: Quantification of a RNA-Virus in several tissues of bank voles Wanted: housekeeping genes
07 July 2018 8,206 1 View
Hello! I am trying to run real-time pcr but whenever the run ends it shows me an error massege " Analysis cannot proceed: not enough samples defined". However I still can get an exponential curve...
07 July 2018 7,384 0 View
Hello Everyone, I have obtained a number of transgenic potato plants via overexpression of one of the genes from defensive pathways to enhance the resistance against the viruses. Then I evaluated...
07 July 2018 3,093 8 View
06 July 2018 9,103 3 View
Hi. I'm doing qPCR on healthy viroid sample. I got N/A or zero value during my amplification but some of my friends got cq value for healthy samples. It make me worried. Healthy sample suppose...
09 June 2018 2,540 3 View
I have qRT-PCR data of 4 transgenic line and 1 non-transgenic line. Non-trangenic line has GOI Ct value zero and HKG has Ct value because non-transgenic has no transgene/GOI, that's why no Ct...
06 June 2018 7,724 4 View
06 June 2018 3,564 3 View
Dear colleagues, I work in a private company, which is serving labs. One of our customer has a serious problem with contamination in the real-time pcr. First contamination was detected in February...
06 June 2018 9,338 3 View