What concentration of the active compound should be used during RT-PCR experiment?

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How is Estrogen receptor alpha distributed in Nucleuoplasm and cytoplasm?

Estrogen receptor alpha is distributed on the plasma membrane, cytoplasm and nucleus. I want to know the differential content of Estrogen receptor alpha in T47D cell lines.

06 July 2017 475 1 View

How to remove non-polar impurity from the compound?

I have done the flash column chromatography of the crude mixture. After the slow elution at 4% Ethyl acetate in petroleum ether, the polar impurity is separated and there is not signal of the...

06 July 2017 5,417 3 View

What are the fellowships available for doing PhD in India?

I am doing Ph.D. in Pharmaceutical Sciences and belong to general category. I have 2-year research experience post M.Pharm. Can someone suggest me the fellowship program from Indian Govt. I know...

01 February 2016 8,668 1 View

What can be possible alternative reagent in following mechanism?

In the attached mechanism, what can be an alternative reagent. In this reaction TBBDA (N,N,N,N-tetrabromobenzene-1,3-disulfonamide) has been used.

05 June 2015 5,554 15 View

Why I am getting double the carbon peaks in spectra?

I have separated the compound using flash chromatography and done 2D TLC chromatography for conforming there is no impurity in the separated compound, but still I am getting double the number of...

04 May 2015 1,987 14 View

How to learn QSAR/QPSAR?

i want to learn the QSAR/QPSAR from the medicinal chemistry point of view, I have gone through many books on QSAR all have a tough mathematical background and calculation which i found a bit...

03 April 2015 2,447 5 View

Is there any difference in the abundance of C-13 during season change ?

has any one seen a change in the abundance of C-13 during the winter and summer season, is it possible to observe relatively high abundance of C-13 in a compound during winter as compared to...

01 February 2015 5,973 5 View

Is there any free software/server available for 4D/5DQSAR?

Many a times we need to study the QSAR of the designed molecules/derivatives. Is there is any free software/server available for 4D/5D QSAR study with a good reliability and results are easily...

09 October 2014 7,571 6 View

How does one separate Benzyl bromide from the reaction mixture?

Benzyl bromide and the product formed are soluble in organic phase like ethyl acetate, the excess of benzyl bromide present in the product may lead to its deterioration. How does one separate...

09 October 2014 5,220 36 View

How a medical practitioner can publish his clinical finding in a journal.

A medical practitioner wants to publish his finding and clinical data of a particular medicinal regimen for a treatment of a disorder. what are the key requirement for the publication. As a...

07 August 2014 8,203 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View