RT-PCR or Luciferase?

More Alex Ortiz's questions See All

How make a series of oil separate into layers?

I want to make a solution in which a series of different oils are mixed (agitated) and then revert to a separate layer looking formation. The same way oil and water by itself would do. I have...

06 June 2017 9,582 5 View

How make a series of oil separate into layers?

I want to make a solution in which a series of different oils are mixed (agitated) and then revert to a separate layer looking formation. The same way oil and water by itself would do. I have...

05 June 2017 4,488 5 View

How to perform DNA extraction from gel where no EtBr was added to visualize position?

I'm trying to establish if a mutation performed on a specific gene worked. I know the size of my gene in question and will eventually send it for sequencing. I don't need to clone it just...

08 August 2016 1,606 4 View

How to perform DNA extraction from gel where no EtBr was added to visualize position?

I'm trying to establish if a mutation performed on a specific gene worked. I know the size of my gene in question and will eventually send it for sequencing. I don't need to clone it just...

07 August 2016 8,567 5 View

What skill-set do you consider, as a professional, a biochemistry undergraduate should have to be considered proficient?

Please don't tell me how you need a Master's because an undergrad is nothing these days. There are some very strong undergrads who have a greater familiarity with a lab setting than many Master's...

06 July 2016 7,158 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why does the MFDFA algorithm need to calculate the profile of the time series?

As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....

05 August 2024 9,366 2 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View

Need help with my research project on open source SIEM and machine learning?

Hello everyone, I am currently working on a research project that aims to integrate machine learning techniques into an open source SIEM tool to automate the creation of security use cases from...

04 August 2024 3,196 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View