Contact experts in Real-Time PCR to get answers
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Questions related to Real-Time PCR
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07 July 2024 5,707 0 View
Effective public communication of research findings requires a combination of skills, including translating technical information, crafting engaging narratives, leveraging multimedia, and actively...
02 July 2024 1,357 2 View
What is the microbial transformation of nutrients in soil and types of changes occur in the amount of energy during its transport from plants to apex consumers?
15 September 2023 4,304 0 View
I am trying the chemical treatment of 3D printed transparent PLA parts by chemical method (solvent dipping) using different solvents. However, a white layer is formed after the chemical treatment...
30 August 2023 8,834 2 View
I am looking to see if anyone has labeled a surface protein and simultaneously confirmed that the cell's plasma membrane is intact and not permeable by probing with a specific antibody used to...
16 February 2023 8,051 1 View
I am doing edge detection of microcell images directly taken form microscope. One image has 25 microwells containing the cells. I am cropping the 25 automatically and trying to do edge detection....
07 December 2022 3,961 0 View
Hi all! I need some help, as I've been trying to troubleshoot and struggling with this for more than a few months. I have DOPC + 05% Texas-Red-DHPE liposomes that I need to fuse into planar...
15 December 2021 3,391 1 View
I tried staining 3D cancer spheroids using F-Actin stain but the F-Actin filament were not well distinguished as lines. It looked like mitochondrial or lysosomal stain. Any ideas why it turned...
06 October 2021 3,788 13 View
Dear collegues, for my research I require of a synteny analysis to locate skin related clusters of a fish specie I am sudying. I have encounter different tools, yet most require coding. I have...
10 June 2021 7,969 1 View
Ratio of Th17/Treg basically??
09 June 2021 5,749 2 View
I have Ct values of my housekeeping gene, and i have ct values of 2 genes of interest. I want to show the level of these genes relative to housekeeping gene and relative to each other. I basically...
26 May 2021 9,530 4 View
I constructed a pseudo ternary diagram and identified the microemulsion range. Then, I formulated microemulsion-based ibuprofen with the ratio of oil/surfactant/cosurfactant in the microemulsion...
15 May 2021 1,127 2 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
02 March 2021 9,272 4 View
I Know that RT-PCR is Reverse Transcription PCR when the starting material is RNA. Going for reverse transcription first (RNA to cDNA) and then it could be conventional PCR with the need for gel...
12 February 2021 7,135 4 View
Hello everyone. I am about to search for the expression of genes including p53 in methylated and non methylated HPV samples. Should I extract DNA from the methylated samples and use SYBR green...
05 February 2021 3,044 3 View
Dear all, I wanted to run HRM ( High Resolution Melting) using Corbett Rotor-Gene 6000 Real Time PCR Machine. When I opened the HRM folder of software , there was two option for running including...
03 January 2021 4,048 6 View
Currently I process a commercial Multiplex Realtime PCR kit on Quantstudio 5 system, but almost 20% of the runs has a distorted amplification plot (see pictures attached below). Does anyone...
15 December 2020 8,047 3 View
High-Resolution Melting (HRM) analysis is a relatively new, post-PCR analysis method used to identify variations in nucleic acid sequences. The method is based on detecting small differences in...
27 November 2020 4,968 4 View
Hi all, I am trying to set up my standard curve for qPCR analysis, presence absence of D. septosporum, using a QuantStudio 5 real-time PCR system and associated software. I am using a 5X...
26 November 2020 930 3 View
I am working on quantstudio 5 system with diagnostic test kit - AZF Microdeletions REALTIME PCR genotyping kit (master mix is aliquoted to every separated pcr tube, I only added taq polymerase,...
20 November 2020 5,074 3 View
I have been using mfold web server for RNA secondary structure prediction, but it is no longer available since the 1st of November. I need a reliable and easy tool alternative to it or a...
03 November 2020 1,120 7 View
I'm a Sr Director of Product Management at Citrix, and I teach Product Management part-time at North Carolina State University (graduate program). I completed my DBA this year and submitted 3...
02 November 2020 7,745 8 View
Hengzhi Shi , Minwei Li , Xiaocui Huang, Chaoqun Yao , Xueqiu Chen , Aifang Du , Yi Yang PMID: 32731054 DOI: 10.1016/j.vetpar.2020.109193.
31 October 2020 9,601 2 View
Hi, I want to multiplex some reaction with dual label probes. I check the probes on FAM, and HEX channel. When I change the dye for ROX channel I don't see any fluorescent. I ordered the probes...
20 October 2020 1,242 5 View