Contact experts in Real-Time PCR to get answers
1,235 views 2,386 posts
Questions related to Real-Time PCR
I got forward and reverse sequence of my target amplicon. i want to assemble them i mean AT and CG .Please recommend me some web applet or bioinformatic software
18 January 2019 4,845 8 View
Dear researchers; A question arose for me about Real-Time analysis. I would appreciate you to comment about the question. For expression analysis we need a housekeeping gene to normalize Ct...
01 January 2019 9,034 4 View
Hello everyone, I need one Geosmin and one 2-MIB producer Streptomyces strain for my PhD thesis for PCR optimisation experiments (both traditional PCR and realtime PCR). Is there anyone to have...
01 January 2019 9,032 2 View
reverse and forward 16S rRNA primary arrangement which can be used to control the number of firmicutes- bactetoidetes with realtime PCR
01 January 2019 6,138 4 View
To assess whether expression of the gene of our interest is associated with mutant status of p53 we have treated FaDu cells( mut-p53) with CP-31398, a Known p53 stabilizer, that restore a...
01 January 2019 8,519 4 View
Can anyone tell me if NALC-NaOH is suitable for liquefaction of sputum for real-time pcr amplification of non TB pathogenic bacteria and virus. Otherwise how to prepare NALC-NaOH or NALC PBS...
01 January 2019 2,752 0 View
Dear All, We work on a project with aims looking at the changes brought about by ketogenic diet( a special form of diet found to be effective in treating drug-resistant epilepsies). We have...
26 December 2018 9,629 3 View
Hello everyone! I want to compare expression of 1 gene (example: Z) between bacteria A and B by quantitative realtime PCR using SYBR green. - First, I made a standard curve for each bacteria by...
12 December 2018 7,695 13 View
is it possible to study NF-kB activity by IKB-a expression? I aim to study IKB-a expression by realtime PCR in order to realize NF-KB activation according to attached article. Is this correct?
12 December 2018 6,085 4 View
12 December 2018 738 4 View
Dear All, We are trying to quantify various bacterial groups in stool samples (DNA) using real-time PCR technique. I would be grateful if anyone can suggest a reference or housekeeping gene so...
12 December 2018 2,376 2 View
Hello, I'm studying for a expression of microRNA in MOUSE granulosa cell. I have used miRCURY LNA miRNA PCR Starter kit for cDNA synthesis and real-time PCR While i was searching for the...
12 December 2018 864 4 View
12 December 2018 8,147 12 View
Our study comprises of PCR analysis of DNA isolated from fecal samples at two time points (at the baseline and after intervention). We have carried out PCR and have obtained the Ct values....
11 November 2018 793 8 View
Hi everybody, I am developing a real-time PCR based animal MHC typing assay. I need to quickly decide whether SYBR Green or TaqMan chemistry should be used. I am focusing on SYBR Green now...
11 November 2018 5,921 5 View
Hi! I would like to know if anyone has the protocol for HPRT (housekeeping gene) conventional PCR (cyles, temperature...)? I've been searching on internet but I just found for real-time PCR. Can...
23 October 2018 8,040 2 View
10 October 2018 9,631 2 View
Hello colleagues, This question has been intriguing me and I have not found a valid answer for it in the literature. I know that exosomes do not have a specific loading control/housekeeping gene,...
10 October 2018 9,427 4 View
Hi, I am currently working on my realtime pcr data. I had analysed my result, but there is something that confused me. Is it possible to have a big fold change (5-fold change) but it is...
10 October 2018 5,938 7 View
Hello everyone! In your experience, changes in the expression of which genes better represent synaptic growth and activity? I look for a marker of vesicular pool (quantity of synaptic vesicles,...
10 October 2018 7,912 4 View
Hi, I have assessed 4 SNPs by TaqMan genotyping assays from Applied Biosystems using StepOne plus realtime PCR system. Now, I tend to analyze my results via TaqMan genotyper software V 1.0.1....
18 September 2018 9,753 5 View
I am working with qPCR on Lactobacillus plantarum WCFS1 and there is a high expression level of 16S rRNA gene comparing to the other genes. Therefore, I am looking for the other housekeeping genes...
09 September 2018 8,552 3 View
09 September 2018 6,975 6 View
Hi I would like to know if mycoplasm infection in cell cuture can alter the real time PCR. tks
09 September 2018 322 0 View