Contact experts in Real-Time PCR to get answers
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Questions related to Real-Time PCR
I'm analyzing the results of SYBR green realtime PCR, but I had difficulty in interpreting the results of one of the genes tested.. As shown below, there was no gene expression in the control...
03 June 2019 5,855 2 View
I am doing real-time pcr with two different markers for the same gene. One marker has amplicon size of 137 bp and the other marker has 343 bp. In results I'm getting difference in their CT values...
07 May 2019 9,178 6 View
My project is about the effect of 2 SNPs in Glut9 transporter and how are these SNPs could be a risk/protective factor in gout. I used real-time PCR for the amplification of SNPs in glut9 gene...
05 May 2019 6,016 1 View
I am optimizing a realtime PCR using probes with FAM and TAMRA. But I do not get any results. The product size is 77 basepairs. Is this small product aize the cause of failed optimization?what...
05 May 2019 8,637 4 View
Hello, we are looking for a real-time PCR machine with the ability to perform HRM analysis, does anyone have any suggestions?
05 May 2019 5,433 1 View
05 May 2019 6,903 7 View
Do we get any differences in assessment of gene expression using primers from many exons of a genes? In general, it is done using single optimised primer in Realtime PCR. What happens if two...
23 March 2019 7,497 3 View
i would like to analyze a qRT PCR (SYBR green) with Real-time PCR Miner Version 4.0. Therefore I need the gene average efficiency, but i have no idea where to find it. The instructions say it is...
03 March 2019 8,352 3 View
Dear researcher, could you please share me your experience? My research is about mirRNA quantitative and qualitative study by PCR. 1. What is optimal miRNA concentration , for the further two...
03 March 2019 6,999 4 View
I want to see global DNA methylation status of samples. What are the different ways by which i can check this? After doing bisulphite conversion, what all genes i can sequence to understand level...
03 March 2019 7,756 4 View
Dear researchers; A question arose for me about Real-Time analysis. I would appreciate you to comment about the question. For expression analysis we need a housekeeping gene to normalize Ct...
28 January 2019 8,626 3 View
I got forward and reverse sequence of my target amplicon. i want to assemble them i mean AT and CG .Please recommend me some web applet or bioinformatic software
18 January 2019 4,855 8 View
01 January 2019 9,040 4 View
Hello everyone, I need one Geosmin and one 2-MIB producer Streptomyces strain for my PhD thesis for PCR optimisation experiments (both traditional PCR and realtime PCR). Is there anyone to have...
01 January 2019 9,045 2 View
reverse and forward 16S rRNA primary arrangement which can be used to control the number of firmicutes- bactetoidetes with realtime PCR
01 January 2019 6,146 4 View
To assess whether expression of the gene of our interest is associated with mutant status of p53 we have treated FaDu cells( mut-p53) with CP-31398, a Known p53 stabilizer, that restore a...
01 January 2019 8,538 4 View
Can anyone tell me if NALC-NaOH is suitable for liquefaction of sputum for real-time pcr amplification of non TB pathogenic bacteria and virus. Otherwise how to prepare NALC-NaOH or NALC PBS...
01 January 2019 2,769 0 View
Dear All, We work on a project with aims looking at the changes brought about by ketogenic diet( a special form of diet found to be effective in treating drug-resistant epilepsies). We have...
26 December 2018 9,645 3 View
Hello everyone! I want to compare expression of 1 gene (example: Z) between bacteria A and B by quantitative realtime PCR using SYBR green. - First, I made a standard curve for each bacteria by...
12 December 2018 7,707 13 View
is it possible to study NF-kB activity by IKB-a expression? I aim to study IKB-a expression by realtime PCR in order to realize NF-KB activation according to attached article. Is this correct?
12 December 2018 6,091 4 View
12 December 2018 746 4 View
Dear All, We are trying to quantify various bacterial groups in stool samples (DNA) using real-time PCR technique. I would be grateful if anyone can suggest a reference or housekeeping gene so...
12 December 2018 2,383 2 View
Hello, I'm studying for a expression of microRNA in MOUSE granulosa cell. I have used miRCURY LNA miRNA PCR Starter kit for cDNA synthesis and real-time PCR While i was searching for the...
12 December 2018 870 4 View
12 December 2018 8,164 12 View