What's the propper way to analise with PCR an artificial feeding medium (sucrose at 15%) for a planthopper for the presence of phytoplasma DNA?

Pablo José Palma Cancino @Pablo_Palma_Cancino

08 August 2017 1 5K Report

After the insect feeds, do you have to threat the medium in any way (use an extraction protocol)? can you analyse it directly (using the medium as template)?

I'll be using a real-time PCR protocol for detection.

Pablo José Palma Cancino

Thanks Mikael! I'll be using an assay which has worked well for us detecting the phytoplasma in plant tissue and the vector's body. I was more concerned about the extraction protocol, I think I'll be using the methodology for extraction reported in:

Lu, H. et al. Determining putative vectors of the Bogia Coconut Syndrome phytoplasma

using loop-mediated isothermal amplification of single-insect feeding media. Sci. Rep. 6, 35801; doi: 10.1038/

srep35801 (2016).

Hope it works.

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