Hi all, Can anybody suggest me the steps required to perform quantitative real-time PCR analysis of mRNA expression data. Actually, I have some differentially expressed genes and I want to validate them by real-time qPCR but I don't have any idea, how to perform real-time qPCR. I have searched the web in this regard but not able to find any suitable answer. If someone please suggest me some good paper to understand that or provide some link to perform this process online then it will be very helpful for me. I am from the computer science background, working in the computation analysis of mRNA data.
Thanks & Regards,
Shib Sankar