Hello everyone!
I want to compare expression of 1 gene (example: Z) between bacteria A and B by quantitative realtime PCR using SYBR green.
- First, I made a standard curve for each bacteria by creating a 10-fold dilution series from a sample with a known concentration of Z gene (standards here are plasmid inserted Z gene of A or B).
- Second, I extracted RNA from bacteria A and B and then treated RNA with a kit . Next, I used the same RNA amount of bacteria A and B to synthesize cDNA
- Third, I did quantitative realtime PCR to quantify Z gene in bacteria A and B. The result are listed below:
Samples Cq Quantity R2 Slope Efficiency Tm
B1 19.8 7.06E+04 0.99 -3.833 82.3 79
B2 18.31 1.10E+05 0.99 -3.833 82.3 78.5
B3 18.83 1.27E+05 0.99 -3.833 82.3 79.5
A1 15.42 2.40E+07 0.98 -3.69 86.7 79.5
A2 14.4 4.52E+07 0.98 -3.69 86.7 79
A2 14.41 4.52E+07 0.98 -3.69 86.7 79
Please help me to know can I use these data to compare expression of Z gene between A and B. If so How to deal with these data?
Thank you.