Protocol: Conventional PCR HPRT gene?

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Water Consumption in Plastic Recycling: Is it Higher Than for Virgin Plastics?

Hello, dear colleagues, I would like your help in finding a reference that provides an average water savings due to plastic recycling compared to the consumption of virgin materials, preferably...

29 July 2024 4,630 0 View

Hello! I would like to transform banana pseudostem fiber into a type of cotton, how can I?

My name is Maria Clara and I'm currently working on my final thesis as a chemistry technician in Brazil. The highlight of my study is to make a biodegradable nappy with a low cost. I would like to...

28 March 2024 847 0 View

Is Native speaker Pronunciation a challenge L2 learners need to overcome?

I am investigating a topic about Native Speaker Pronunciation. I am looking for any resources related to this topic. I am curious whether students should still be motivated to obtain it or perhaps...

02 March 2024 7,375 10 View

¿How do I measure the angle of a Closterium spp?

Is it measured from the apical parts to the center of the microalgae ? or do you have another ways to measured it? I attach an image to explain thanks

12 February 2024 6,924 3 View

How to determine IC50 significance?

Hi guys, I am performing MTT assays to charactherize tumor cells resistance to chemotherapy (I am comparing the treated cells vs. control (non treated) from different tumor cell lines). I have...

31 January 2024 2,623 4 View

How can I solve this problem?

When I tried to prepare a solution of enzalutamide of 1000 uM in cell culture medium from a solution of 1 M of enzalutamide in DMSO the drug precipitated. I've tried changing the pH and the...

08 December 2023 3,947 1 View

PTFE as a binder for Lithium ion batteries cathode, how to ?

Numerous articles mention the combination of metal oxide, carbon black, and PTFE as a binder, followed by pressing onto an Al mesh. Yet, I encounter difficulties in achieving uniform pellets using...

07 December 2023 4,298 3 View

Individual-level career shocks?

I am investigating career shocks, and using Seibert et al. (2013, 2016) scale. However, these account for shocks that happen within the organizational setting, and I am also interested in...

06 December 2023 4,890 0 View

RNA extraction from liquor samples??

Hi, guys Have you ever extracted RNA from liquor (cerebrospinal fluid) samples? Do you have a protocol to share? In the lab, we normally use the QIAGEN RNeasy kit. Do you think it will work?...

11 November 2023 3,695 4 View

Can corticosterone:HBC complex be stored once reconstituted in water?

Hi all, I have just prepared CORT:HBC (sigma) in water, and I did not used all the solution, do you know if it can be stored once is reconstituted, and for how long? Thanks in advance, Renata

05 November 2023 5,543 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why does the MFDFA algorithm need to calculate the profile of the time series?

As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....

05 August 2024 9,366 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View

Need help with my research project on open source SIEM and machine learning?

Hello everyone, I am currently working on a research project that aims to integrate machine learning techniques into an open source SIEM tool to automate the creation of security use cases from...

04 August 2024 3,196 2 View