Dear Nanik,

You may refer to this published study: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4561689/

You can also design your own based on your specific needs.

Hope this helps.

All the best,

Arizaldo

@Arizaldo Castro

thank you for the information, but in the recommended journal it turned out that the sample was an animal (mouse) could the 16S rNA applied on the control for human faeces / stool to find out the number of firmicutes-bacteroidetes ?

Dear Nanik,

16s rRNA gene is used to identify bacterial isolates using gene sequence similarity and the source of your bacterial isolate does not really affect the RT-PCR process. I think it will still work. Perhaps you can check other published reports, too.

-Arizaldo

@Arizaldo Castro

Thank you for the information.

I have to get the Journal for primary 16S rRNA in human as a control for firmicutes bacteroidetes.

RESEARCH ARTICLE

Methods for Improving Human Gut

Microbiome Data by Reducing Variability

through Sample Processing and Storage of

Stool

Monika A. Gorzelak, Sandeep K. Gill, Nishat Tasnim, Zahra Ahmadi-Vand, Michael Jay,

Deanna L. Gibson*