I got forward and reverse sequence of my target amplicon. i want to assemble them i mean AT and CG .Please recommend me some web applet or bioinformatic software
Seqscape is a very good software to use. Im guessing what you have are ABI files that you want to turn into FATSA files ??
You can use Cap3 sequence assembly program to assemble the forward and reverse reads from Sanger sequencing of your target amplicon.
Weblink of cap3: http://doua.prabi.fr/software/cap3
It is very simple to use
1. Convert the .ab1 files to fasta format
2. Add the forward and reverse reads in this sequence into the cap3 assembler
Cap3 assembles the reads into a consensus sequence. The page refreshes once the results are ready to be downloaded.
You can blast the result in NCBI for confirmation.
Hi,
Tracy might suit your needs. It is open source and also has web app as described here:
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6635-8
Each of the following programs could be appropriate:
BioEdit (https://bioedit.software.informer.com/7.2/)
Phred-phrap (http://www.phrap.org/phredphrapconsed.html)
DNA Subway (https://dnasubway.cyverse.org)
Geneious Prime
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