MspI/HpaII enzyme digestion in genomic DNA samples following LUMA analysis can estimate the global methylation level (see attached slides). LUMA analysis does not involves bisulfite modification.

If bisulfite modification based method is used, Line-1, Alu, LTR12C, or other repetitive elements can be used as global methylation markers.

If you are interested in getting information in gene level, WGBS, RRBS, and EPIC array are bisulfite based methods. Hope this helps.

Hi, I did something fun like this recently, and I had an idea for getting such an information.

I have 4 differant methylation sensitive enzymes, use them parrallelly, plus an undigest.

design an illumina primer that target a consensus sequence related to the area you are looking at

take your digested DNA and cut with a non methylation sensitive restriction enzyme and add a linker for rad-seq

Run them parralelly, on illumina and padam! you know that if a long enough fragment is missing somewhere, it is overmethylated!

It seemed that the repetitive elements, such as Line, Alu, or LTR, were sequenced. High level of methylation in repeats is require the genomic stability.

Global DNA methylation could be assessed using several methods including simple ones like ELISA and flourimetry.

If you are interested in bisulfite conversion- based methods in particular, LINE-1 and Alu are well studied markers.