I want to distinguish neutrophils from other cells in PFA-fixed and paraffin-embedded tissues. Could you provide recommendations for a basic histology staining method, other than H&E staining, that would be suitable for this purpose?
Actually it is not so difficult to distiguish neutrofils from other cells because of their prominent polymorph nuclear structure , segmented and stick-formed nuclei. You can try a Giemsa-staining. This could help you.
One alternative staining method you could consider is immunohistochemistry (IHC) using specific antibodies against neutrophil markers, such as CD15 or myeloperoxidase. IHC allows for the visualization of specific cell types within tissue sections and can help distinguish neutrophils from other cells in PFA-fixed and paraffin-embedded tissues. This method can provide specific and detailed information about the distribution and localization of neutrophils in the tissue.
Can be easily identified in H and E stained sections because if their multilobed nucleus and granular cytoplasm morphology. iHC can be done using CD10, CD15 and other markers for better differentiation.
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