Hi,
Is it possible to measure the protein concentration after digestion with Proteinase-K e.g. using BCA method? When compared to homogenized samples without Proteinase-K, Proteinase K digest produced increased absorbance during an experimental setup. So, I am curious to know the possibility or the interpolation of data.
Best
Debashish
Hi Deb,
I think you can not measure protein concentration after adding proteinase-k, because the enzyme interferes your total protein measurement.
but I guess you can measure the protein concentration of proteinase-k before adding to the samples and eventually subtract the number from total protein concentration.
Good luck
It sounds like you started with a homogenized sample of cells or tissue and found that, after treatment of the homogenate with proteinase K, the protein concentration was higher. This is a reasonable result. In addition to the proteinase K protein itself, as Omid pointed out, the treatment will have liberated peptides from the homogenate that may not have been accessible to the BCA reagent prior to the treatment.
You can measure protein with & without proteinase K and follow the Lowry's method
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