Is there any chemical or surgical method to induce hypoxia in mouse model?

Dear all, My question is about Inducible hypoxia in vivo (mouse model). I'm looking for some chemicals that can induce the pathway of hypoxia or some other techniques. If you now know something...

15 December 2020 866 6 View

Acetic acid in liquid waste ?

Hy, may you suggest a method to quantify acetic acid in wastes or leaching test? Thanks in advance Stefano

10 December 2020 4,023 2 View

Which points I have to take in mind to mutate a plasmid that grow in Neb stable strain?

Several kits that allow the introduction of point mutations in plasmids say to transform mutated plasmids in DH5alpha bacteria. I have a plasmid with many repetitions that need stable strains to...

08 November 2020 4,250 2 View

Did you have any problem for staining nuclear phosphorylated proteins in hippocampal neurones?

I was wondering if there may be a problem of permeabilisation of cells. I am working on mice hippocampus (pups) and I need to make a staining for a phoshoprilated nuclear protein. Since the...

17 September 2020 2,000 3 View

Glass or PVC material to perform a soil leaching experiment of pharmaceuticals?

Hello I am developing a soil column experiment to study pharmaceuticals transport and attenuation.I would like to know If is better to use a glass or a PVC column to perform this experiment and...

10 August 2020 6,214 3 View

How many researchers are interested in attending web communications in the field of Sustainable Planning?

17 June 2020 4,753 74 View

What happens to non-"preclincial essential research" (non Covid19) in Covid times?

Are you askiing what researchers are doing in Covid time especially those that work with animal? I started a survey to collect information (anonymous) to see whether there are alternatives to...

02 April 2020 9,596 3 View

Generic DC/HYBRID grid-connected microgrid available in simulink?

I'm looking for a microgrid simulation model written in simulink in order to implement my simulation data and test my Energy Management System algorithm. The microgrid I am looking forward is a...

24 October 2019 3,541 4 View

Is there an investigation about the real world implementation of decision making algorithms in smart home/microgrids EMS microcontrollers?

Implementation of stochastic and probabilistic based algorithm is a very tricky task especially in energy management systems microcontrollers. I'm looking for a research paper which talks about...

30 July 2019 5,947 1 View

What is the best choice for a ribosomal protein used as a loading control in WB?

Hi everyone. I have to assess protein expression in my adult satellite muscle cells by WB. I'd prefer not to use the conventional beta-actin or alpha-tubulin, nor GAPDH. I saw that 18S mRNA was...

24 June 2019 3,133 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View