I want to know simple method of assay and related substance analysis for Amino acid. If anyone have experience of analysis of these then please let me know. I need this method.
In our lab they use HPLC or UPLC. This is a simple and useful method as there are many different forms of chromatography one can use, depending on the headgroups of your amino aids of interest, that will yield acceptable results..
What makes this a useful method is that one can use an array of varying detection techniques, from MS/MS to radiolabel detectors. this wiil once again depend on the amino acid of interest, and also the desired resolution.