Optimally, it would contain data from a sample for several instruments tracking performance over time, possibly with annotation.
Yes, as far as I am aware they are here:
(1) MS1-based label-free proteomics quadrupole Orbitrap mass spectrometer for Escherichia coli digest spiked into a HeLa digest in four different concentrations is made available, deposited to ProteomeXchange with identifier PXD001385 (Shalit et al., 2015).
• Shalit, T, Elinger, D, Savidor, A, Gabashvili, A. and Levin, Y, 2015. MS1-based label-free proteomics using a quadrupole orbitrap mass spectrometer. Journal of proteome research, 14(4), 1979-1986.
(2) Sigma UPS1 48 protein mix (all equimolar proteins) spiked into a Yeast digest background at different concentrations using a Orbitrap Velos running High-Low (FT for MS1 and CID ion trap MS/MS scans) and is made available as PXD001819 at PRIDE DB (Ramus et al., 2016).
• Ramus, C, Hovasse, A, Marcellin, M, Hesse, A.M, Mouton-Barbosa, E, Bouyssié, D, Vaca, S, Carapito, C, Chaoui, K, Bruley, C. and Garin, J, 2016. Benchmarking quantitative label-free LC–MS data processing workflows using a complex spiked proteomic standard dataset. Journal of proteomics, 132, 51-62.
(3) SWATH-MS Gold Standard data set were made available (Röst et al., 2014) to the proteomics research community.
• Röst, H.L, Rosenberger, G, Navarro, P, Gillet, L, Miladinović, S.M, Schubert, O.T, Wolski, W, Collins, B.C, Malmström, J, Malmström, L. and Aebersold, R, 2014. OpenSWATH enables automated, targeted analysis of data-independent acquisition MS data. Nature biotechnology, 32(3), 219-223.
Thanks,
Biswa
Thank you very much for your answer. These are great data sets. I was more looking for a series for a QC sample acquired one one or multiple instruments to monitor its performance state.
Hey Hi,
May be this paper can answer your query at least for SWATH MS on Triple TOF.
https://www.nature.com/articles/s41467-017-00249-5
- Badges
- Science topic
- Similar topics
- Chemistry
- General Biochemistry
- Proteomics