Dear all,
I am wondering if someone uses a single point or double point method to determine the intrinsic viscosity of their proteins.
These methods are usually developed for polymers and does not fit for proteins.
Could you share with me your experimence on this.
Best,
Sébastien
Dear Sébastien Brûlé, literature data shows a wide use of these techniques. Please check the following documents. My Regards
https://www.researchgate.net/publication/225546165_Determination_of_intrinsic_viscosities_of_macromolecules_and_nanoparticles_Comparison_of_single-point_and_dilution_procedures
https://doi.org/10.1021/ct600062y
https://doi.org/10.3933/applrheol-19-53347
DOI:10.1007/s11095-017-2112-8
https://doi.org/10.1155/2013/360239
Below an element of reflection.
From polymer theory the intrinsic viscosity is directly proportional to the reverse of the internal concentration i.e. C*=M/R^3, where M is the molecular weight and R the radius of gyration. For polymer chains with fractal dimension D, C*~M^(1-3/D). Globular proteins are compact (D=3) so C* is constant and also the intrisic viscosity. Thus the technics seems a priori to be a little bit inappropriate for proteins, I mean no systematic dependency is expected but rather some variations from one molecule to another, which do not contain a lot of informations.
Can you please give more details about what you are looking for ?
Kindly check this link
Intrinsic viscosity determination by "single-point" and "double-point"
"Single-point" equations used for intrinsic viscosity ... To overcome it we propose a "double-point" method which ... tion to molecular conformation , Adv. Proteín.
https://www.researchgate.net/publication/286968827_Intrinsic_viscosity_determination_by_single-point_and_double-point_equations
Determination of intrinsic viscosities of macromolecules and ...
Comparison of single-point and dilution procedures ... The determination of the
https://www.researchgate.net/publication/225546165_Determination_of_intrinsic_viscosities_of_macromolecules_and_nanoparticles_Comparison_of_single-point_and_dilution_procedures
In order to evaluate the intrinsic viscosity, the relative viscosity – using an appropriate viscometer – as well as the concentration c need to be determined. The latter is conventionally determined using either a spectrophotometer for proteins or a refractometer for any macromolecule.
https://link.springer.com/10.1007/978-3-642-16712-6_283
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