Hi Everyone,
I am trying to characterize the microstructure (localization of carbohydrates, fats, and proteins) of coconut milk powder using confocal microscopy. From the review of the available literature, I have found that fats and proteins can be non covalently labeled using fluorescent dyes. However, I am having trouble deciding the labeling protocol for the carbohydrate. Two common methods that I have seen in the papers are as follows:
1. Use antibodies or lectins conjugated with fluorophores to label carbohydrates
2. Covalent labeling of carbohydrates with FITC
If I use the first method, the signal from antibodies or lectins may interfere with the signal from protein present in the sample.
In the second method, the FITC (probe used for covalent labeling) also shows an affinity for proteins. So, I am afraid that the use of such a probe may also interfere with the protein signal (from the food sample). Is there any other probe available that I can use?
What would be the best way to label carbohydrates such that I can simultaneously image three components in the food mixture?
Thanks.
Dear Sheetal!
You look at this publications, please:
Article Using Confocal Laser Scanning Microscopy To Probe the Milk F...
Article Organization of lipids in milks, infant milk formulas and va...
https://hal.archives-ouvertes.fr/hal-00929674/document
Confocal microscopy is a promising technique to identify the microstructure of multiple components (Carbohydrates, fat, proteins) in food such as cheese, milk or mayonnaise, hazelnut oil, or olive oil.
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