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Hi, I am trying to delete genes in Streptococcus mutans using PCR ligation mutagenesis following published primers of the target gene locus and Spectinomycin cassette. The study used pDL278...
08 June 2023 7,645 2 View
Do you have any references that have properties of different desiccant materials? For example, we can replace Lb: the latent heat at surface temperature in Eq. (36) in the paper Talaat, M. A.,...
07 June 2023 9,163 0 View
Dear researchers, I am currently researching the issue of fracture in thin steel plates containing holes. Some scholars have already conducted static tensile fracture tests on plates with holes...
05 June 2023 3,546 1 View
As it's well known, breast cancer can be caused through genetics factor. How did it happen? Does it have corelation with epigenetics? And what are the chances that it can be lowered?
05 June 2023 3,489 3 View
Can it be posited that the presence of a severe boundary layer separation, characterized by the detachment of the fluid flow from a solid surface, serves as a significant contributing factor to...
04 June 2023 7,330 23 View
I have a list of plasmid sequences but I don't have primers. I thought that I could use the ORF sequence for the design, however, the primers are not specific. Can anyone tell me how to design a...
02 June 2023 610 4 View
For the Gibson cloning into pH-ePPE vector (19kb), I use NEB Hifi builder mix with 400ng of vector backbone (18kb) and 10ng of 250bp insert and NEB chemically competent 10beta cells for...
02 June 2023 1,025 4 View
I am specifically interested in breast cancer cells but numbers for any cancer type would be very helpul. Also what is their typical diameter?
02 June 2023 4,694 1 View
I am working with PEN membrane slides used in laser capture microdissection. When I try to cut the cells on the membrane, too many bubbles are seen. I made a tiny hole on the edge of the membrane...
01 June 2023 9,245 3 View
What causes the Shrimp larval rearing tank culture water turns into GREEN COLOR ? which has (live Thalassiosira weissflogii microalgae& industry standard Probiotic & other growth...
01 June 2023 2,216 2 View
Are there any established optimal ranges for Lipoprotein (a) in adults?
01 June 2023 1,153 0 View
Can abiotic factors impact organisms in each biome & nitrogen from atmosphere the abiotic part of ecosystem converted into biotic part of ecosystem in organisms?
31 May 2023 6,293 1 View
I'm working on my thesis about reliability analysis of composite structures. Can I use maximum deflection of the plate structure as failure criteria to define limit state function to perform...
31 May 2023 5,226 1 View
I'm a PhD researcher in my first year and I need some help about my question
31 May 2023 3,492 2 View
Hi For the validation purpose, I need some published data for upward flow between parallel plates in mixed convection
30 May 2023 1,783 2 View
I'm interested in your previous experience with growing diatoms in plexiglass PBRs. I intend to use the photobioreactor for monospecific cultures/experiments with Skeletonema costatum. It has a...
30 May 2023 7,921 3 View
How can R programming be used for interactive data visualization and exploration in research? What are some recommended R packages and techniques for creating interactive plots and dashboards?
29 May 2023 2,331 3 View
Pls tell me in details process..I am familiar with cell culture, tissue culture as well..
26 May 2023 8,946 0 View
Our colleagues are preparing for their 3D spheroid culture and they plan to use 1~1.5% agarose solution to coat the plate. When choosing the agarose for use, there are different kinds to agarose...
25 May 2023 5,742 2 View
I want to know which software is used by authors to draw schematic diagrams of beams, plates etc. for there articles.
23 May 2023 7,627 6 View
I'm making chalcone using claisen condensation of aldehyde and acetophenone, piperidine as base in EtOH solvent. But after 2-3 days of refluxing my reacamt is not consuming. There is product spot...
23 May 2023 5,069 11 View
I mixed bacterial colony in PBS from a fresh plate. now i know bacteria cannot grow in PBS. But when i mix it in broth will it grow bacteria?
20 May 2023 9,031 4 View
Hello everyone! I need some help with a Generalized Linear Mixed-effect Model. Here's the problem: In my GLMM, I have two cluster variables (Stimuli and Participants) that I have modeled as...
19 May 2023 5,511 4 View
Dear scientists I'm trying to make an experimental study on solar air heaters with a selective coating Aluminum abosrber plate with 0.7 mm thickness but I'm facing a problem with the plate where...
17 May 2023 8,573 0 View