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Hello everyone, I recently had my MS machine cleaned since then i am facing an issue regarding implementation of my method for ascorbic acid using Sequant ZIC HILIC-MS/MS. I see a break in my...
05 August 2023 8,600 1 View
Hello all, In CV QKD, in general, there is two noise sources in the system. the shot-noise, which is the fundamental noise of the signal and arises from quantization of the electromagnetic...
03 August 2023 2,050 5 View
Did anybody actually check the pH before and after autoclave? My different medias are starting to be acidic after autoclave and I can't find an explanation for this observation?
30 July 2023 9,286 8 View
suppose, 1microM of analyte is added into the water sample and then used for testing. Then how to calculate the concentration of analyte found in the final solution?
29 July 2023 1,801 0 View
We extracted DNA from feacal samples and now we need to transport them from Botswana to the Netherlands for downstream procedures. Does any one know the procedures that are required to adhere to...
28 July 2023 9,688 0 View
when there's a development of harmful algal blooms which produce toxins such as microcystin, anatoxin, saxitoxin which hamper the ecosystem and due to algaecide treatment these toxins are...
27 July 2023 9,366 1 View
Hi! Could someone help me to solve this problem? I will coat the 6-well plate with anti-CD3 first. Then, add anti-CD28 into T cell culture and remove T cell culture into 6-well plate. However,...
24 July 2023 1,013 1 View
I isolated and differentiated bone marrow-derived macrophages from mice. After differentiation I frozen them and kept them in liquid nitrogen. Then, I thaw them and let them recover for 3-5 days...
24 July 2023 9,730 4 View
I am searching for a validated tool on Pressure Injury Prevention for patients undergoing surgery
22 July 2023 3,892 1 View
Dear community, i need your help concerning one issue i have in geometrical modeling in ANSYS. I need to built up in APDL my model ( see the picture) using solid rail with real profile instead...
22 July 2023 2,192 6 View
Hi, I want to extract lipid rafts by using an Optiprep gradient in order to analyze some receptors. After ultracentrifugation and recovery of the fractions, I used TCA (trichloroacetic acid) to...
19 July 2023 364 0 View
Hello everyone! Is there any standardized assay or procedure to measure optimum working conditions (mainly temperature and pH) of lipases and/or phospholipases? What substrates and buffers do you...
19 July 2023 9,500 0 View
I am planning to perform intracellular Ca2+ mobilization assays on 96-well plates using EndoC-βH1 cells. Specifically, I am interested in measuring β-cell responses to glucose and possibly other...
17 July 2023 3,931 0 View
Material or any composite that have least friction between its sirface and air , which decrease s the boundary layer and also delay boundary layer separation..
16 July 2023 8,840 1 View
Dear all I have modeled Viscous damper in ETABS using link but when I perform TH analysis and view the display cumulative response, the energy in VD remains zero. I may me making some mistakes in...
15 July 2023 9,541 0 View
The cell line I used in my experiment is a self-cultured porpoise fibroblast cell line. On the first day, I seeded cells on a 96-well plate and the cell density was 5 x 10^4 cells/mL. The day...
14 July 2023 6,813 5 View
Hi! Are there any R packages or Python modules that are able to implement the COW algorithm in chromatogram alignment? Thanks!
14 July 2023 1,979 0 View
Do you know how to solve differential equations by using Bernstein operational matrices?
13 July 2023 3,167 0 View
"primers at a concentration of 10 µmol/L" "primers at a concentration of 10 mmol/L" "primers at a concentration of 0.5 μmol/l" I found this concentration from three different article. How...
12 July 2023 1,572 7 View
Hello, Does anyone have information about the ploidy of HT22 mouse hippocampal? I couldn't find anything about in the literature. Thanks!
12 July 2023 1,157 2 View
Why does the laser for optogenetic stimulation need to be shuttered on and off rapidly? Can i use a constant laser light but at low power?
12 July 2023 2,627 3 View
Hello Everyone!!! I am trying to differentiate RAW264.7 cells (1million cells/1ml/ well of 12well plate) using 10ng/ml or 50ng/ml of RANKL concentration for 5 to 7 days. Later on the TRAP staining...
11 July 2023 4,081 1 View
decrease of fetuin A and increase of serum osteocalcin used as vascular calcification in CKD patients .
11 July 2023 7,553 1 View
Items that purport to be "Related research" are completely unrelated. My item on Diptera is matched against medical cardiovascular research - what? If the word "newsletter" is in your title then...
10 July 2023 9,538 1 View