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Questions related to Plating
Hi, does anybody know if there is an endogenous nucleus marker specific for glutamatergic neurons which can be used to purifie nuclei from mouse brain by sorting? Thank you!
22 August 2022 9,546 1 View
Does anyone have any experience with long-term storage/freezing of CFSE-stained bacterial cells and their later use in experiments? Do you notice any difference in the fluorescence levels from the...
22 August 2022 7,037 1 View
My advisor and I recently extracted high-molecular weight DNA from Acanthamoeba polyphaga using Cells and Tissue DNA Isolation Kit (Magnetic Bead System) by Norgen Biotek Corp. Our objective is to...
17 August 2022 5,726 0 View
I am very worried about EHP.
11 August 2022 3,507 3 View
I work on generalized Navier-Stokes equations with FEM and B-spline. I want a matlab code to solve this type of equations by this method.
01 August 2022 794 1 View
recently I got some problems with my spreading of agar plates. 1. The first plate on 7.8 got nothing in PCR (we did a clone transformation) but I think the major problem is from ligation....
28 July 2022 6,495 0 View
I have referred to this paper " Design and research of hybrid piezoelectric electromagnetic energy harvester based on magnetic couple suction-repulsion motion and centrifugal action", 2021. In...
27 July 2022 7,883 0 View
I am looking to gently shake a 96 well plate with 100uL of cell suspension per well to keep the cells from settling on the bottom.
27 July 2022 4,217 0 View
I recently made some dockings with certain substances and obtained negative values of "VINA Score" but I don't know how to interpret it.
12 July 2022 2,876 3 View
Dear all, i am currently working on Li plating mechanism in 3D Carbon substrates. I use Cu foil as the control electrode. During plating/stripping of the Li-Cu half cell, the cell experienced a...
06 July 2022 562 0 View
For Raman/PL peak position mapping corresponding to a particular peak via wire software we need to do the curve fitting first and after the fitting we do the mapping. For my case after cureve...
29 June 2022 9,719 0 View
I am having a research about Porphyridum cruentum, and i would like to adapt it from sea water media to fresh water media. If you have knowledge about it, I will be so appreciate if you would like...
26 June 2022 8,637 0 View
When the recombinant E. coli was cultivated in a 3L fermenter, the growth of the bacteria was normal before induced by lactose, and the parameters such as pH and DO were normal. But about 5 h...
24 June 2022 1,876 3 View
Left read files: $VAR1 = [ 'SRR14294419_1.fastq' ]; Right read files: $VAR1 = [ 'SRR14294419_2.fastq' ]; Saturday, June 18, 2022: 23:36:39 CMD: mkdir -p...
18 June 2022 2,480 0 View
I am doing a research about polyycrstal MAPbI3, and my recepit is follow byYC. Kim et. al. The main porcess include: Equivalent molar ratios of MAI and PbI2 in 22wt% γ -butyrolactone in sealed...
16 June 2022 4,551 1 View
Hello all, does anybody know which one is suitable to consider residual stresses: ANSYS APDL or ABAQUS? Then how to add it? Your valuable comments make me happy. Thank you
11 June 2022 2,225 1 View
I need the calculation required to produce 1 h2 kg/hr using 1 MW power supply in a PEM electrolyser. I want to calculate the Input Current Voltage Water Required for 1 h2 kg/hr Thank you in...
01 June 2022 5,513 2 View
Thinking about getting astrocytes derived from IPSc. So I read that first both astrocytic like cells and neurite like cells grow together and to purify astrocytes 3 passages are made.My question...
31 May 2022 6,793 2 View
good morning, I have a problem of repetibility for my IC50 on spheroid. Indeed I am trying to determine the IC50 on a 3D culture in spheroid. I am doing a range of 10 concentrations in...
31 May 2022 5,594 0 View
I would like to knock out a specific gene of Vibrio Cholerae. I am aware of the Transflp method but I wonder whether an even more simple method exists.
28 May 2022 2,728 1 View
Initially I thought It may be due to the air trapped bubbles during spotting of OP50. I thought If I allow the bacteria to grow in 37 degree celsius incubator, the bacteria might cover the...
27 May 2022 1,167 0 View
I need information regarding the X-Ray Micro-CT facility in India on an urgent basis. Thank you.
26 May 2022 6,163 4 View
dear all i want to detect the cytotoxicity of CTL on the tumor cells. i plan to sort normal mouse CD8 T cells from spleen and lymph node by Magnetic bead, and then coculture with B16F10 cell line...
25 May 2022 8,648 0 View
Has anyone else dealt with static electricity on Arabidopsis plates? The static causes the seedlings to root upwards. Passing your finger along the top of the plate causes the upward root to...
02 May 2022 3,173 2 View