Decreasing pH after autoclave?

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Murugan Thirumalai

There are less likely pH will change in media if we autoclave. Sugars like glucose are not allowed to autoclave. May be this could be because of autoclaved sugars.

Golnaz Sadeghne

Thanks for your answer, Actually my medias are SCDA, FTM, ATM and SCDM. They are ready to use. I only weight them and solve them in purified water. Before autoclave everything is OK, But after autoclave the pH is changing to acidic point and I can't understand the reason behind this observation!!

Overheating may also be the reason so that carbon dioxide may expel out. If pH raises to alkali, try adding a drop of sterile HCL after autoclaving.

Yes, I think so, how can I check the autoclave procedure to see whether it is working well or not🤔🤔🤔

Bacillus stearothermophilus vial can be procured. This vial should be attached to the wall inside of the autoclave. After sterilization, the vial should be kept incubated at 55 C for five days. If colour changes noticed, then the autoclave is not working good.

So I should try to find this bacteria. Thanks for your help.

We have autoclave label that confirms the sterilization is okay but you told something about degrading sugars inside of the autoclave. Do you think this bacteria will be a good testament for proper autoclave procedure?!!

Bacillus stearothermophilus is a biological indicator. It is used to check if autoclave reaches 121 C and 15lb pressure. At this temperature indicator strain dies. But the media turns to acid is due to changes in protein or sugars in your media. Therefore, kindly use the me

Now I am confused 🙄The bacteria can be died if temperature reaches above 121 so it couldn't be a good way to check the proper autoclave procedure. Do you know what I am saying?