Plating

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Understanding PCA plot for MD simulations ?

In this figure, a pca plot is shown for different protein , protein complex systems depicting the essential subspace for these systems. I want to know how can I interpret these plots ? What...

01 April 2024 10,093 3 View

Does anyone know of any published research work that addresses the topic of using rhizobacteria that promote plant growth through foliar inoculation?

I am interested in knowing if there is any precedent in the scientific literature on the use of plant growth-promoting bacteria obtained from the rhizosphere that have been inoculated into the...

25 March 2024 6,611 2 View

When a project fails, what is your biggest concern - when it comes to losses?

I am trying to understand the pressure leaders might feel, when a project fails. It is undoubtedly a moment when we should, stop-rethink-process-and move on. But often times since a large amount...

19 March 2024 1,312 0 View

How many murine Monocytes and naive BMDM (Macrophages) should I adoptively transfer before an osteotomy?

I want to image via 2-photon microscopy immune cells after an Osteotomy. I will adoptively transfer murine monocytes and macrophages. What would be a good number to transfer/cell type? And has...

15 March 2024 8,979 0 View

Can anyone provide a protocol for mitochondria isolation for western blot ?

Currently I am trying abcam protocol for it.i checked the cell lysis by trypan blue stain which is more than 90 %. but I am facing following issues 1. unable to remove beta actin in mitochondrial...

11 March 2024 7,566 1 View

How to predict excess enthalpy using cosmo-rs?

Dear Dr, Currently i was studying to extract pyrrole from n-hexadecane using protic ionic liquid( triethylammonium salicylate). When i compute the excess enthalpy formula it shows vdw is higher...

05 March 2024 5,708 0 View

Does Backward elimination work to identify potential factor?

Hi everyone, I would be appreciative if you could give your suggestion on my goal: My goal is : To determine the factors that can predict Anxiety disorder(AD) and Quality of life(QOL) in the...

01 March 2024 3,793 0 View

Can you please orient me to bibliography concerning financial planning about public health insurance?

27 February 2024 7,143 0 View

I need help accessing this article: DEBELLUM” - Lower limb multilevel treatment with drug eluting balloon - randomized trial: 1-year results ?

https://www.minervamedica.it/en/journals/cardiovascular-surgery/article.php?cod=R37Y2014N02A0207

23 February 2024 9,571 1 View

How can I isolate Chemolithotrophs from domestic water tank?

I am working on microbiome of extreme environment for my master's thesis. If anyone has an idea please let me know

20 February 2024 5,086 0 View

What are the origin of crystals that form upon heating castor oil?

In the process of maleinizing castor oil with maleic anhydride I am observing formation of crystal-like structures upon heating the solution above 100 degrees Celsius. The solution is just castor...

03 February 2024 5,224 1 View

Can you please suggest a free AI reader?

online

03 February 2024 6,070 2 View

Is it possible to vaporize molybdenum wire and prepare a thin film of it on the substrate by flash evaporation method?

For making a thin layer of Mo

25 January 2024 5,892 3 View

How to solve the large deformation Jacobian matrix in ABAQUS : DDSDDE?

In large deformations, UMAT is very strict in deriving Jacobian matrices. And there are many tangential stiffness matrices in the book that I don't know which one should be? Do you have friends...

18 January 2024 3,817 0 View

How do we determine the type of supercapacitance behaviour shown by a material using GCD curve?

Does iR drop play a role in the type of supercapacitor (EDLC, Pseudo or hybrid)?

13 January 2024 2,087 1 View

What potential by-products may occur in the EDC/NHS reaction?

I want to use carboxymethyl cellulose for an EDC/NHS reaction to attach amine-functionalized small molecules. Initially, I used an EDC:NHS ratio of 1:1, but the grafting efficiency was low. Later,...

12 January 2024 6,990 1 View

Can the substrate be larger than the heater in a Spray pyrolysis machine?

Probably a very simple question, but one I can't find an answer to. If a spray pyrolysis unit has an oven of 800mm x 860mm and a heater inside of only 250mm x 250mm, can a 400mm x 500mm sheet of...

12 January 2024 8,651 1 View

What are the benefits of finite element analysis in road construction?

what are the benefits of finite element analysis in road construction?

11 January 2024 3,884 4 View

Why the Mg element is rarely found in high-entropy alloy designs?

A very interesting phenomenon, for the existing high-entropy alloy design, the use of more elements are Fe, Ni, Co, Cr, Mn, Al, V, Ti, Zr, etc. However, the Mg element is rarely found in HEAs....

10 January 2024 2,735 4 View

Is there any software available for designing primers which can take multiple sequence input as fasta format?

I would like to design primers for 18S rRNA gene of Cryptosporidium spp. Usually, I use NCBI to design primers. Then I check the primers with multiple sequences alignment in MEGA or design the...

10 January 2024 576 5 View

What is the impact of a Journal changing publishers for an author of a research article?

Dear all, I submitted a research manuscript to the "Journal of the Geological Society of India" on May 25, 2023. The paper underwent two rounds of "Major Revisions," which were successfully...

09 January 2024 2,908 0 View

Max Pages limit for publicaton in EURASIP Journal on Wireless Communications and Networking ?

Hi, can anyone tell max page limit for publication in "EURASIP Journal on Wireless Communications and Networking" ? . Also, is this limit for double line space (review) or final publication ?

08 January 2024 1,699 1 View

What is meant by relaxing the system while taking EIS ?

While reading the literature I came across a statement that we need to relax the system before taking Impedance measurement. What does it mean?, How to make the system in relaxed state and how to...

07 January 2024 3,531 3 View

How long would you treat macrophages with nigericin? And would you harvest them right after treatment?

I am planning to activate inflammasome in macrophages and epithelial tumor cells. I was wondering if I could harvest macrophages right after nigericin treatment (for example 4 hours treatment then...

07 January 2024 575 0 View

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