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Questions related to Plating
How to calculate size of the alignment In this case
23 November 2023 3,509 1 View
I want to preserve tissue samples to use them in DNA extraction for epigenetic and methylation analysis. How can I preserve samples for DNA extraction and methylation analysis?
22 November 2023 5,102 0 View
Hello dear researchers, Do you know how to solve and prove the formula 5.27 (The Volume Expansion Coefficient for Low Solute Concentration) mentioned in the "diffusion mass transfer book" by...
19 November 2023 294 2 View
I recently started working with 16s metabarcoding and I just encountered a situation I don't know it's common or not. I'm mostly working with the bioinformatics part of the project. We have 5...
17 November 2023 2,466 1 View
this answer needs to be comprehensive
15 November 2023 8,148 1 View
What is the coldest layer of the earth and which layer of the atmosphere contains the ozone responsible for the absorption of ultraviolet light?
12 November 2023 5,795 0 View
How does the rhizosphere affect crop productivity and how rhizospheric microorganisms are the gateway to a sustainable plant health?
11 November 2023 6,891 4 View
What is the effect of plant root on rhizospheric microbes and role of plant growth promoting rhizobacteria for improving crop productivity in sustainable agriculture?
11 November 2023 3,107 3 View
I am using lectin-based ELISAs to probe the glycans of different glycoproteins. I had successful data that showed clear differences in the lectin binding profiles/patterns. However, I wanted to...
09 November 2023 1,625 0 View
In the process of using patterned photoresist as a mask and RIE to etch metal thin film layers, there are often problems with incomplete etching or over etching resulting in pattern disappearance
08 November 2023 3,162 2 View
I am using ELK-FPLAPW code to compute band structure of some materials. From the output files, I can plot l (or m_l) resolved band structure using Origin-Pro. But since I have the license of this...
07 November 2023 9,227 3 View
VUMAT subroutine which describes cyclic constitutive model of steel bars which tell us the ultimate strain bars using FORTRAN language for ABAQUS user defined material.
05 November 2023 9,576 2 View
I have been trying to determine Km and Vmax of alpha amylase with the MM plot but I cannot reach the saturation point. My substrate concentration (starch) goes from 0.1 to 3 mg/ml and in my last...
03 November 2023 8,197 3 View
Dear Friends, I have a big problem defining a Hinge or (pin) joint between 2 separated discrete rigid which are both shell surfaces and one of them should be assembled with the rest single...
03 November 2023 8,046 2 View
I tried to find a suitable standard for mechanically testing epoxy resins but I keep finding standards that direct me to other materials such as rubbers or cements/mortars. I was interested in...
01 November 2023 5,066 8 View
We are trying to measure degree of conversion of resin composite under ceramic restoration using FTIR. The specimens are flat and are cured next to a cellophane strip, so they are smooth and...
01 November 2023 8,457 1 View
I am having trouble understanding the different approaches where pull down vs co IP is used. I have to study protein- protein interactions associated with a specific protein. I have developed an...
31 October 2023 2,640 4 View
Hi all, I am simulating a flow over a cylinder using LES. Looking at several academic papers on the same topic, statistics are calculated after several vortex shedding cycles. I would like to...
30 October 2023 703 7 View
I've been looking into 1-3 piezoelectric composites lately. I am particularly interested in the process of making them with high aspect ratio. However, I have come across conflicting opinions in...
30 October 2023 4,875 0 View
I am trying to minimize the gate leakage current of a FET devices and found that hBN is a good dielectric for 2D materials (MoS2, WS2) based FETs. I was curious if we can deposite it by e beam...
30 October 2023 7,209 0 View
I work in a project where we need to knockout a gene at primary T cells targeting an exon that is shared between all variants. However, while the sgRNA design has been optimised ,we still see low...
25 October 2023 5,715 1 View
Hello, we need to sterilize borosilicate made transparent glass vials with gamma radiation (15 or 25kgray). Question 1: Do these glasses discolor after radiation? Question 2: Does this...
24 October 2023 1,725 0 View
Made a lot of CsI films with Tl doping by vacuum evapration, did SEM and ICP and other optical test, but never find out what is the key effect of CsI afterglow. Need some suggestion for next step.
23 October 2023 5,187 0 View
Dear all, We have a landfill which is filled with sludge containing high amount of Arsenic (approximately 412,000 ppm). This sludge is the byproduct of a acid factory wastewater treatment plant...
16 October 2023 9,895 2 View