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Questions related to Plating
Density
04 April 2023 9,466 5 View
In many of the crops it is noted one or the other colour of the fruit is considered as a dominant character. In many of the crops masking or co- dominant character is also noted. How it is...
04 April 2023 8,110 1 View
I am working on pseudomonas aeruginosa. My bacteria is susceptible to antibiotics, However will it be able to form biofilms?
30 March 2023 8,407 3 View
The PMFS a sinistral plate boundary fault system that occurs in eastern Guatemala. It accommodates the motion caused by the movement of the North American Plate and the Caribbean Plate.
28 March 2023 6,228 0 View
Hi, I have a few questions regarding MTT assay with treatment as it is my first time doing it. 1. After seeding cells in 96-well plate, do I need to remove the existing media from it before I...
23 March 2023 5,806 3 View
Hi all, I have a crack in plate which extend under fatigue load using XFEM . I can measure the length of crack. My problem is how to get number of cycles to draw curve between length and number...
16 March 2023 6,377 0 View
How nanofertilizer and nanopesticides are applied for improvements in crop production and protection and what are Nanofertilizers in smart agriculture?
16 March 2023 7,688 5 View
I am getting a thin white layer in my lymphocyte culture. I used dmem media with 10% fbs to culture lymphocytes. After culturing 24 hours in CO2 incubator, i observed a thin white layer formation...
15 March 2023 6,585 1 View
I'm trying to find experimental evidence that confirms diaphragmatic spasm is the cause of inspiratory apnea after a person gets winded (blow to the upper abdomen). Amazingly, this appears to...
15 March 2023 5,435 0 View
My column is fairly new and clean and operates well in other HPLC instruments, my method is a gradient elution method, and my solvents are ACN and water. I have removed the column and set a union...
14 March 2023 1,070 4 View
I have a cell culture of DLD1 cells and I need to seed them into a 6-well plate for RNA isolation. Please clarify me whether the following procedure would work or any other recommended method to...
13 March 2023 3,420 10 View
Both the Bell and Kern methods provide correlations for predicting the heat transfer coefficient and the pressure drop in the case of the shell-and-tube heat exchanger design. Would those...
09 March 2023 4,615 4 View
I‘m reading about research ‘Surface-Tension-Dominant Crystallization of 2D perovskite single crystals for vertically oriented hetero-/homo-structure photodetectors’. I understand it until in...
07 March 2023 3,414 1 View
Hi everyone, I'm working on the isolation of bacteriocin from lactic acid bacteria. I've a bacteria that I isolated and know to be lactobacillus. When I cultivated this bacterium in liquid culture...
05 March 2023 836 18 View
I am thinking of ANSYS and COMSOL multiphysics. Also considering the following list. Commercial software 1) Simcenter STAR-CCM+ 2) Battery design studio (BDS) 3)Thermal desktop Open source...
03 March 2023 8,284 5 View
How can I calculate the dose of the antimicrobial agent for poultry if I know just the MIC of that antimicrobial agent?
02 March 2023 7,105 4 View
In the biofilm test assay, the methods states to use polystyrene Microplates. But does not specify which kind rather non-binding, low-binding, medium-binding, or high-binding. Which is the best...
27 February 2023 4,826 3 View
The aim of my project is to identify Cathepsin S inhibitors using in silico models and in vitro through fluorescence-based enzyme assay, and tissue culture. Atherosclerosis is a chronic...
25 February 2023 9,942 6 View
Greetings from IIT Bombay ! We cordially invite you to participate in International Workshop (offline only) on 3D Lithography and Bioprinting (3DLitho-2023) to be held on 4th and 5th April 2023....
23 February 2023 8,986 1 View
I am currently struggling to get non-adherent or dead hepatocytes out of the system after seeding them into a microfluidic chip. I have already tried to increase cell viability prior to seeding...
23 February 2023 1,823 1 View
I am working on parabolic trough receiver optimization using solar tracing software TracePro. I don't know how to obtain the flux distribution on the surface as an x-y chart. any advice ? Regards,
23 February 2023 635 0 View
am performing an ELISA method to determine EC50 binding of several glycan binding proteins against IgG (it is N-glycosylated at N297 in the Fc region). I am having difficulties in the ff finding...
22 February 2023 4,907 0 View
How often does this happen in the laboratory?
22 February 2023 4,147 4 View
When transferring culture in petri dish from one place to another, what should we do to avoid contamination of the bacteriological work?
22 February 2023 7,572 5 View