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Questions related to Plating
Dear experts, I have recently conducted a Rietveld refinement using FullProf for a compound based on A cellulose I-beta. However, I encountered some issues with the results. The parameters U, V,...
07 July 2023 1,102 1 View
We're making an E. coli intracellular expression product. In one batch of fermentation, OD was reduced by half within one hour, and phage contamination was found by post-electron microscopy....
06 July 2023 9,392 2 View
I tried to record electrophysiological activities (eg: action potential) of neurons from cortical organoids at 100-day-olds using whole-cell patch clamp. I was able to get the giga seal easily....
04 July 2023 1,226 5 View
I was trying to use Cytoscape to replace IPA to fit our labs convenience, also with no self-generated database or profiles I feel the need to start with the Biogateway app, however whenever I...
04 July 2023 9,525 0 View
As we know at the end of welding near the plate edges, the temperature rises too much i.e. the maximum temperature in the arc region is higher than all other points. Now, I am looking for a...
03 July 2023 3,517 2 View
I am trying to evaluate stress levels in different developmental stages of rats. In order to do so I need to measure corticosterone in their fecal, urine, or blood samples. Since we are short on...
03 July 2023 3,839 4 View
I think these phenomena follow the scientific facts defined in the classical mechanics, but not theories or definitions in Einstein's mechanics.
02 July 2023 1,406 9 View
If using an electric heating plate for digestion
29 June 2023 9,995 1 View
Under anaerobic conditions organic acids are secreted into the culture media. Also corynebacterium glutamicum is known for producing aminoacids. I am not sure if the color change from yellow to...
29 June 2023 1,187 1 View
The numbness was due to the injury of inferior alveolar nerve during extraction of lower right third molar and using high speed hand-pace also that the numbness continue several days. I want know...
29 June 2023 4,151 5 View
I want to perform MTT assay for BJ-5ta and HaCat cell lines. I want to know before performing the actual experiment how many passages should be done of the cell lines?
27 June 2023 5,562 3 View
Is it plausible that cells exposed to an oxidative stressor would display increased metabolism (as measured through MTT assays)?
26 June 2023 7,165 2 View
What polymers containing phosphorus are used in maxillofacial surgery? List all such polymers. Thank you!
25 June 2023 6,205 1 View
Hello! I really hope anyone can help me out. For my master thesis I am comparing cancer cell clones and their reaction to certain targeted inhibitors. Now I would like to perform a two way ANOVA...
24 June 2023 7,434 1 View
Hello, I'd like to include some background for the problem I'm having. I'm working with Candida glabrata isolates in 96 well plates. My plate conditions are: 1*10^6 cells/mL, 5%CO2, 24h...
23 June 2023 8,516 1 View
I am currently conducting research on the development of filaments comprising 25wt% graphene, 25wt% carbon nanotube, and 50wt% polylactic acid (PLA). However, I have encountered an issue where the...
22 June 2023 4,832 2 View
Hello, I have been trying to linearize a vector through PCR in prep for cloning for several weeks now. I am using Inverse PCR as opposed to enzyme restriction digestion. However, every time I have...
21 June 2023 6,800 1 View
I am working with iPSCs and at a certain point when dividing them, they differentiate into neurons for no apparent reason since they are treated following the same division protocol. Could someone...
21 June 2023 2,622 1 View
What phosphorus-based polymers, including 3D printed ones, are used in dentistry? What works contain information about them? Thank you!
20 June 2023 6,532 0 View
Dear All Does any body help me who to add to create multi-nodes in one zone in Type 56 in TRNSYS? Many thanks in advance
19 June 2023 3,465 3 View
I am working on a project to use bacterial fermentation of proteins from plant-based resources. I was wondering if anyone comment on which lactic acid bacteria i.e. Lactobacillus helveticus or L....
19 June 2023 804 1 View
my question is that after the successfully culturing of bacterial colonies on LB agar plates after 2 hours incubation at 37 degree celsicus. how long agar plates can b stored on same temp with...
19 June 2023 7,615 2 View
An error is showing during the verify all members passed in SAP2000 for a G+3 RC Hill building. The bottom columns of dimension 400x400 mm2 while remaining columns dimension is 300x300 mm2. The...
16 June 2023 4,106 1 View
Why measurement under 630nm is too low around 0.7（Pi is 10nm in the well）, whether the color of solution is normal ? Is it necessary to add Oxalic acid? Malachite green reagent 1.2% malachite...
15 June 2023 9,438 3 View