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Questions related to Plating
What can be the meaning of this sentence? "Because the real area of a rough surface is bigger than its nominal area."?
13 May 2023 5,135 2 View
I left complete RPMI and DMEM media (with FBS and Pen) overnight at 37 Degree water bath. Can I use these?
12 May 2023 1,443 3 View
I am trying to use BriteVu casting reagent to perfuse the coronary vasculature and image using microCT. I have tried 1) perfusion via catheterization of the descending aorta with ligation of...
12 May 2023 2,578 1 View
Do you know if the ElISA plates have an expiration date?
10 May 2023 7,327 1 View
Hello, What is the surface tension of Sodium chloride (NaCl) 0.9% (saline solution)? At air/liquid interface At 25 °C (and 20 °C) Thank you in advance for your help 😊
10 May 2023 4,214 3 View
How to determine the DNA and protein damage in bacteria caused by antimicrobial agents?
10 May 2023 1,505 2 View
Hi any one guide me that on congo red agar my no strain showed black color. among all showed the pink or colorless although optical density showed the biofilm forming strain what i do please help me.
10 May 2023 9,049 0 View
TLC profile
08 May 2023 8,898 1 View
I want to construct the basis functions for P and Q elements
08 May 2023 9,355 3 View
Hello! I am curious , can anyone guide me how we can calculate the amount of hydrogen is stored in the metal hydride during the absorption process both in %wt. an in grams and how much energy is...
07 May 2023 3,201 2 View
Hello All I have a question regarding real-time (RT) Primers Optimization. We usually perform gradient-PCR with standard PCR conditions for primer optimization for qualitative PCR. But for...
06 May 2023 8,855 2 View
Dear all, I need help now. I run HPLC with one of the peptide, on RP with 0.1% TFA/ACN as mobi phases. There is an impurity peak came out. The weird thing about this peak is 1) did not show in my...
06 May 2023 794 7 View
Hi, I am trying to simulate piezoelectric energy harvester in COMSOL an followed the COMSOL Multiphysics manual as a guide. Only difference was I worked in the 3D plane instead of 2D. I have...
05 May 2023 9,492 2 View
I am trying to evaporate a 1mm thick CsI:Tl scintillator. It works fine with CMOS, while it unusually increases its radioluminence around 1-3% when X-ray is giving. The temperature effect was...
04 May 2023 9,657 1 View
I have a few questions about sintering of metal nanoparticles which I would like to get some help about. I am trying to sinter titanium nanoparticles after electrospraying them. My questions...
03 May 2023 7,511 2 View
Are all good and benefit bacteria considered as probiotics?
02 May 2023 3,885 2 View
Hi Is there any criteria value for banded (microstructure) rate?
30 April 2023 8,474 1 View
often when I document I find in the articles a ratio of absorbances of which I don't know how they determined the two values of this ratio and what is really the use of calculating it
29 April 2023 6,880 2 View
Can you please help derive an equation for Rau with respect to the other parameters from this circuit? Thanks a lot!
28 April 2023 8,417 2 View
I need to buy immortalized BBB endothelial cells like bEnd.3. Where may I buy it within Japan? If you have experience please suggest me.
27 April 2023 8,688 1 View
I have to clone a gene below its native promoter but it is part of an operon, so I need to amplify the two genes separately and clone it in two steps. I found a plasmid in my lab which already has...
24 April 2023 6,504 5 View
I tried to do DFT calculation but the submit button was disabled. I cannot do any other calculations also
23 April 2023 1,646 3 View
May some of you performed staining for immunofluorescence of PBMCs in 96-well plate? As the cells are not going to be cultured, only stained in these plates for confocal microscopy, we are...
22 April 2023 1,997 3 View
I am looking for software that can be used by healthcare students studying clinical assessment to learn anatomy and techniques of musculoskeletal assessment within a virtual environment. Most of...
22 April 2023 5,596 6 View