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I am trying to isolate the intracellular proteins from fungal biomass in a filamentous fungi (P. sporulosa, cell wall has more glucans) for proteomics study. I am facing problem in the sample...
22 February 2023 5,955 2 View
According to enzymatic method for extracting extracellular DNA in biofilm matrix, is suggested to use CTAB solution. Why? I only need extracellular DNA... Do you have a similar protocol to...
21 February 2023 2,290 1 View
I ran a batch culture and measured OD at 600 every half hour. I need to make a graph of growth rate /h. How to convert OD values to growth rate values at each point? I am attaching the graph I...
20 February 2023 9,680 1 View
I am trying to upload a file and get an "error parsing server response". How do I solve this?
14 February 2023 7,330 9 View
Hi. I have formalin fixed tissue and FFPE tissue from the same samples which have been in storage for 1-2 weeks. I am looking to obtain nucleic acids for qPCR, where the target is 80bp. Is there a...
13 February 2023 7,928 4 View
I am confused about how to calculate the Reynolds Number (Re) of the largest eddies in a turbulent flow. It seems that many discussions I read (university course notes, for example) state that...
13 February 2023 9,166 4 View
I‘m trying to culture lymphocytes. I’m attaching the pictures of the media that are available to me. kindly help me out. How do I supplement FBS with the RPMI-1640 media that is available to me? I...
13 February 2023 2,731 5 View
I would like to design an experiment where primary hepatocytes from mice have to maintained in culture for about a week. However, I don not know how long the primary hepatocyte can survive in...
12 February 2023 7,661 4 View
Explain your ideas
11 February 2023 1,545 6 View
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09 February 2023 3,880 6 View
I am planning an MDA-MB-231 xenograft experiment for a drug efficiency study. Based on the literature review, I am planning to inject 0.8x106 cells in 100ul PBS into the 4th mammary gland of BalbC...
08 February 2023 6,498 4 View
I have used Ethanol and potassium hydroxide to dissolve Tantalum. But it didn't work. Anyone have the idea , how could it be dissolved properly?
08 February 2023 1,089 0 View
Hello, we have case study about a design of a truss used for PV panels and we wanna determine if the design of the truss will withstand the PV's weight and the wind forces. What is the criteria...
07 February 2023 8,173 3 View
I did a MTT assay for a plant extract against L929 cells. But in readings, the cell viability percentage was increased more than the untreated cells, with increasing concentration.
01 February 2023 2,601 1 View
Good Day Based from your experience, I would like to ask if it is usual that there are dead and floating transfected HEK293 cells after seeding in PLK-coated coverslip? In my experiments, I used...
30 January 2023 2,010 1 View
These types of figures are required in reaction kinetics modeling work. #figure #plot #Histogram #reaction_kinetics #chemical_kinetics #combustion #combustion_kinetics
30 January 2023 453 7 View
I am facing problems with NEB Stable Cells. The original stock has an expiry date of 2020 but we thought the glycerol stocks would be fine. We are not able to clone and transform in NEB competent...
29 January 2023 8,943 3 View
I am qualitatively trying to determine the sialic acid using resorcinol on TLC plates. Can someone help me with the process?
27 January 2023 7,821 0 View
I am looking for temperature dependent Young's (elastic) modulus data for Kovar. Could anybody help with that?
27 January 2023 2,953 0 View
I suspect the protein I'm trying to detect is sensitive to SDS in my lysis buffer. Of course, I could shorten the lysis duration but it's not a good option for several reasons. Does anyone...
27 January 2023 4,035 2 View
Hi all, I've recently upgraded from the Agilent Seahorse XF24 to the XFe24 and have started having issues with the OCR calibration. The rep suggested that storing the hydrated cartridge at 4C even...
27 January 2023 3,258 3 View
When BSC is added on the standards (Hippuric Acid and pyridine), formation of yellow-colored complex is observed with increasing intensity as the concentration of Hippuric acid is increased....
27 January 2023 4,616 0 View
Sample anti realist perspectives **Fields are just cognitive constructions that provide countrrfactuals to reason observe behavior of charged particles ** When there is no motion, forces do not...
21 January 2023 5,101 2 View
What is the best software to simulate heat transfer inside a receiver tube of parabolic trough solar collector?
18 January 2023 3,714 5 View