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Hi, I want to extract lipid rafts by using an Optiprep gradient in order to analyze some receptors. After ultracentrifugation and recovery of the fractions, I used TCA (trichloroacetic acid) to...
19 July 2023 338 0 View
Hello everyone! Is there any standardized assay or procedure to measure optimum working conditions (mainly temperature and pH) of lipases and/or phospholipases? What substrates and buffers do you...
19 July 2023 9,475 0 View
I am planning to perform intracellular Ca2+ mobilization assays on 96-well plates using EndoC-βH1 cells. Specifically, I am interested in measuring β-cell responses to glucose and possibly other...
17 July 2023 3,906 0 View
Material or any composite that have least friction between its sirface and air , which decrease s the boundary layer and also delay boundary layer separation..
16 July 2023 8,818 1 View
Dear all I have modeled Viscous damper in ETABS using link but when I perform TH analysis and view the display cumulative response, the energy in VD remains zero. I may me making some mistakes in...
15 July 2023 9,515 0 View
The cell line I used in my experiment is a self-cultured porpoise fibroblast cell line. On the first day, I seeded cells on a 96-well plate and the cell density was 5 x 10^4 cells/mL. The day...
14 July 2023 6,795 5 View
Hi! Are there any R packages or Python modules that are able to implement the COW algorithm in chromatogram alignment? Thanks!
14 July 2023 1,948 0 View
Do you know how to solve differential equations by using Bernstein operational matrices?
13 July 2023 3,147 0 View
"primers at a concentration of 10 µmol/L" "primers at a concentration of 10 mmol/L" "primers at a concentration of 0.5 μmol/l" I found this concentration from three different article. How...
12 July 2023 1,558 7 View
Hello, Does anyone have information about the ploidy of HT22 mouse hippocampal? I couldn't find anything about in the literature. Thanks!
12 July 2023 1,134 2 View
Why does the laser for optogenetic stimulation need to be shuttered on and off rapidly? Can i use a constant laser light but at low power?
12 July 2023 2,590 3 View
Hello Everyone!!! I am trying to differentiate RAW264.7 cells (1million cells/1ml/ well of 12well plate) using 10ng/ml or 50ng/ml of RANKL concentration for 5 to 7 days. Later on the TRAP staining...
11 July 2023 4,052 1 View
decrease of fetuin A and increase of serum osteocalcin used as vascular calcification in CKD patients .
11 July 2023 7,528 1 View
Items that purport to be "Related research" are completely unrelated. My item on Diptera is matched against medical cardiovascular research - what? If the word "newsletter" is in your title then...
10 July 2023 9,513 1 View
Dear experts, I have recently conducted a Rietveld refinement using FullProf for a compound based on A cellulose I-beta. However, I encountered some issues with the results. The parameters U, V,...
07 July 2023 993 1 View
We're making an E. coli intracellular expression product. In one batch of fermentation, OD was reduced by half within one hour, and phage contamination was found by post-electron microscopy....
06 July 2023 9,321 2 View
I tried to record electrophysiological activities (eg: action potential) of neurons from cortical organoids at 100-day-olds using whole-cell patch clamp. I was able to get the giga seal easily....
04 July 2023 1,138 5 View
I was trying to use Cytoscape to replace IPA to fit our labs convenience, also with no self-generated database or profiles I feel the need to start with the Biogateway app, however whenever I...
04 July 2023 9,434 0 View
As we know at the end of welding near the plate edges, the temperature rises too much i.e. the maximum temperature in the arc region is higher than all other points. Now, I am looking for a...
03 July 2023 3,451 2 View
I am trying to evaluate stress levels in different developmental stages of rats. In order to do so I need to measure corticosterone in their fecal, urine, or blood samples. Since we are short on...
03 July 2023 3,743 4 View
I think these phenomena follow the scientific facts defined in the classical mechanics, but not theories or definitions in Einstein's mechanics.
02 July 2023 1,267 9 View
If using an electric heating plate for digestion
29 June 2023 9,897 1 View
Under anaerobic conditions organic acids are secreted into the culture media. Also corynebacterium glutamicum is known for producing aminoacids. I am not sure if the color change from yellow to...
29 June 2023 1,092 1 View
The numbness was due to the injury of inferior alveolar nerve during extraction of lower right third molar and using high speed hand-pace also that the numbness continue several days. I want know...
29 June 2023 4,058 5 View