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Questions related to Plating
I want to perform MTT assay for BJ-5ta and HaCat cell lines. I want to know before performing the actual experiment how many passages should be done of the cell lines?
27 June 2023 5,408 3 View
Is it plausible that cells exposed to an oxidative stressor would display increased metabolism (as measured through MTT assays)?
26 June 2023 7,009 2 View
What polymers containing phosphorus are used in maxillofacial surgery? List all such polymers. Thank you!
25 June 2023 6,060 1 View
Hello! I really hope anyone can help me out. For my master thesis I am comparing cancer cell clones and their reaction to certain targeted inhibitors. Now I would like to perform a two way ANOVA...
24 June 2023 7,257 1 View
Hello, I'd like to include some background for the problem I'm having. I'm working with Candida glabrata isolates in 96 well plates. My plate conditions are: 1*10^6 cells/mL, 5%CO2, 24h...
23 June 2023 8,423 1 View
I am currently conducting research on the development of filaments comprising 25wt% graphene, 25wt% carbon nanotube, and 50wt% polylactic acid (PLA). However, I have encountered an issue where the...
22 June 2023 4,733 2 View
Hello, I have been trying to linearize a vector through PCR in prep for cloning for several weeks now. I am using Inverse PCR as opposed to enzyme restriction digestion. However, every time I have...
21 June 2023 6,673 1 View
I am working with iPSCs and at a certain point when dividing them, they differentiate into neurons for no apparent reason since they are treated following the same division protocol. Could someone...
21 June 2023 2,501 1 View
What phosphorus-based polymers, including 3D printed ones, are used in dentistry? What works contain information about them? Thank you!
20 June 2023 6,404 0 View
Dear All Does any body help me who to add to create multi-nodes in one zone in Type 56 in TRNSYS? Many thanks in advance
19 June 2023 3,380 3 View
I am working on a project to use bacterial fermentation of proteins from plant-based resources. I was wondering if anyone comment on which lactic acid bacteria i.e. Lactobacillus helveticus or L....
19 June 2023 672 1 View
my question is that after the successfully culturing of bacterial colonies on LB agar plates after 2 hours incubation at 37 degree celsicus. how long agar plates can b stored on same temp with...
19 June 2023 7,506 2 View
An error is showing during the verify all members passed in SAP2000 for a G+3 RC Hill building. The bottom columns of dimension 400x400 mm2 while remaining columns dimension is 300x300 mm2. The...
16 June 2023 3,991 1 View
Why measurement under 630nm is too low around 0.7（Pi is 10nm in the well）, whether the color of solution is normal ? Is it necessary to add Oxalic acid? Malachite green reagent 1.2% malachite...
15 June 2023 9,281 3 View
How the gene cloning work and mention few steps?
15 June 2023 8,464 2 View
Phage enriched in SM buffer and 10x enrichment bacteriophage broth produced crystals upon storage. Can any one tell me the reason for this?
14 June 2023 7,521 4 View
While forming polymer gels by crosslinking PHPA and PEI at 80oC or higher temperatures, the gel surface starts turning yellow in some days, and eventually the entire gel turns yellow and gets...
14 June 2023 1,078 0 View
At what temperature will entropy become constant and why does entropy always increase?
14 June 2023 7,015 5 View
Does anyone know whether there are any manufacturers still producing photographic emulsion for microautoradiography? My understanding is that the previous manufacturers are no longer making...
13 June 2023 7,676 0 View
Bioelectric mechanism - the essence of the appearance of an electronic signal in response to the appearance of bacterial cells in the sample. What is the reason for the generating of the...
10 June 2023 3,729 0 View
the error: An error occurred Error parsing server response.
10 June 2023 8,809 0 View
because what I know, is that gonorrhea culture requires at least 2-5% CO2 gas.
09 June 2023 540 3 View
The complete combustion of octane can be used as a model for the burning of gasoline: 2 C8H18 + 25 O2 : 16 CO2 + 18 H2O Assuming that this equation provides a reasonable model of the actual...
09 June 2023 8,558 1 View
Any kind of suggestions based on Sol-gel.
09 June 2023 7,019 1 View