How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

What is suitable temperature at reaction mixture of lipase activity assay?

I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...

04 February 2021 9,100 5 View

How can I produce Alpha-monopropionin and Alpha-monobutyrin in lab scale?

I understand that glycerol should be reacted with propaoiocic acid to produce monopropionin and with butyric acid to produce monobutyrin , if you can help with the reaction conditions based on 1...

13 January 2021 1,151 4 View

Insect Lipase Activity?

Hi everyone! My name is Nacho. I am trying to assay lipase activity from insect midgut supernatant, and I have some problems and some doubts about it. I do not have the pH-stat. Thus, the...

22 November 2020 5,595 3 View

Why do researchers use amylase and lipase from porcine pancreas?

I found that many researchers use amylase and lipase from the porcine pancreas to research the inhibitory activity of those natural extracts. Why they choose amylase and lipase from the porcine...

17 November 2020 4,672 8 View

How to I determine the esterification activity of a lipase?

Hi, I need help to find a fitting method to determine the esterification activity of a lipase. I am using the Novozym 435 and I am also using a very small volume in this reaction. The enzyme...

22 September 2020 5,904 6 View

Why the absordance of positive control and negative control of lipase activity assay seems the same?

I am doing the lipase activity assay using 4-nitrophenyl dodecanoate as lipase substrate and lipase from Aspergillus oryzae(L4277). 4-nitrophenyl dodecanoate was dissolved in 100mL 5mM Sodium...

14 May 2020 1,497 4 View

How are digestive enzymes for human consumption commercially produced?

Hello, Many protein shake powder mixes contain digestive enzymes such as protease, lipase, and cellulase. However, the products do not detail from which organism the proteins are produced or...

21 March 2020 5,048 3 View

Can someone help me with Plate assay for Lipase production screening please?

I came across Kouker and Jaeger wok using olive oil. Needed help on that.

24 February 2020 4,589 3 View

Can someone help me with Plate assay for Lipase production screening please?

I came across Kouker and Jaeger wok using olive oil. Needed help on that.

01 February 2020 2,847 3 View