What is the best way to precipitate protein after ultracentrifugation in Optiprep gradient?

Carolina Cuevas Mendoza @Carolina_Cuevas_Mendoza2

19 July 2023 0 291 Report

Hi,

I want to extract lipid rafts by using an Optiprep gradient in order to analyze some receptors. After ultracentrifugation and recovery of the fractions, I used TCA (trichloroacetic acid) to precipitate proteins, however, the pellet (only visible in the first and second fractions) is yellowish-colored; and the last two fractions turned out yellow with a suspended solid (as seen in the picture). Do you know why is this happening? Any idea about what this solid is?

Finally, after TCA treatment, which solvent (different from acetone) could I use? Optiprep is not soluble in acetone.

Thanks in advance,

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