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Questions related to Plating
Anyone who has worked with culturing of lymphocytes, can u plz tell me that after I isolate the lymphocytes and put them in a CO2 incubator, I need to see if my lymphocytes have grown and whether...
18 January 2023 1,908 2 View
Is it difficult to stabilize an intrinsically disordered protein by MD Simulation? What is the solution for this?
17 January 2023 183 1 View
The GPT CHAT is starting to gain momentum on the web, everyone is talking about it, and we have all, or almost, tried it. Should we see this technological advance of the I.A is an opportunity to...
14 January 2023 604 5 View
We subjected a bioactive glass material to antimicrobial studies and derived various zone of inhibition values. So we will like to know range of values that's good before concluding it potent as...
14 January 2023 1,330 7 View
I do expression of Fc Fusion proteins in CHO and HEK293 cell in 24 well plate. On day 4 or 5 cell viability decreased less then 50%. After purification i get 10 time less proteins then the CRO.
12 January 2023 5,611 7 View
Dear respected all I want to work on 76 story benchmark building problem. I want to know still the problem exist or not? Because I have tried to get problem MATLAB code from SSTL 2002 for third...
12 January 2023 1,691 1 View
In particular oversampling is done on train dataset, in order to prevent data leakage in test dataset, as of Undersampling, I am not clear how to proceed? Doing Undersampling before train test...
05 January 2023 888 4 View
05 January 2023 1,701 1 View
I am looking for Microbial Protease Inhibitor. HOWEVER. It has to be specific to bacteria. (it is in human stool samples which have both Microbial and Human Proteases. All Inhibitors I found are...
04 January 2023 8,849 1 View
I have been culturing human astrocytes derived from brain samples. Do not have any problem growing them on T25, T75, and multi-well plates (Poly D lysine coated). But, when I grow them on...
03 January 2023 9,751 5 View
Dear all I am working on 76 story benchmark building with dampers. can anybody help me to download MATLAB code for 76 story benchmark buildings from SSTL 2000. I have tried 3rd generation code for...
03 January 2023 9,319 0 View
Do all Heliozoa have to have axopodia, or can they just comprise of overlapping silica plates?
02 January 2023 418 0 View
I need to freeze dry cyanobacterial solution, but I don't have acces to freeze dryer. Can I freeze my solution and use "sodium hydrxide solid form" to absorb the moisture?
25 December 2022 4,346 5 View
I am performing molecular dynamics simulation in GROMACS for a protein-ligand (Phytochemical) complex using GROMACS via Simlab (Online). Currently they provide only 50 ns simulation. I need 100...
24 December 2022 2,105 4 View
Hi everyone, I am trying to find literature about a molecule that binds to an inhibitor. I am not sure whether it is also a type of an inhibitor. I am specifically searching if someone has found...
20 December 2022 5,079 1 View
I want to make sure that my competent cells are NEB stable cells only but I couldn't find the genome sequence or NEB stable specific primers on web. Has anyone ever tried to validate their NEB...
17 December 2022 8,447 2 View
Hello, I want to use a multiple(2) fragments In-fusion cloning technique, so I tried to design four primers with snapgene and takara online tool, but all of the primers have a Tm more than 65 °C...
15 December 2022 6,505 4 View
We want to reduce TDS of aqueous solution. We have explored membrane technology and crystallization. Now we are looking to explore biomass for TDS removal. Pease suggest suitable bacteria.
12 December 2022 4,577 7 View
How to compute a homogenous mixing two materials? Is the following general formula of calculate effective conductivity yelds considerable result? Keff=V1 K1 +(1-V1)K2
11 December 2022 8,820 3 View
hello i have a question about the cloning i am trying to do the cloning for ECFP and EYEP , although i have colonies on my AMP plate the result of my cloning is negative. would you please help me...
07 December 2022 5,864 3 View
Is momentum equation in gas dynamics an advanced form of Bernoulli's equation? Please explain in detail.
05 December 2022 7,926 3 View
Hello all, My name is Matt, and I am a 2nd year PhD student whose work currently focuses on intestinal gene expression. In brief, to collect intestinal tissue samples, we harvest sections of the...
05 December 2022 7,140 4 View
Hey all I noticed that when I forgot to passage HeLa cells after 10 days the media colour had changed to yellow and I could see colonies everywhere. I gave multiple 1X PBS wash and passaged them....
03 December 2022 2,064 4 View
After performing a Western Blot from a previously run acrylamide gel onto a nitrocellulose membrane, I have added a primary antibody solution (in BSA blocking buffer) and incubated the membrane...
02 December 2022 277 4 View