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Questions related to Plating
I have been trying to determine Km and Vmax of alpha amylase with the MM plot but I cannot reach the saturation point. My substrate concentration (starch) goes from 0.1 to 3 mg/ml and in my last...
03 November 2023 8,243 3 View
Dear Friends, I have a big problem defining a Hinge or (pin) joint between 2 separated discrete rigid which are both shell surfaces and one of them should be assembled with the rest single...
03 November 2023 8,102 2 View
I tried to find a suitable standard for mechanically testing epoxy resins but I keep finding standards that direct me to other materials such as rubbers or cements/mortars. I was interested in...
01 November 2023 5,106 8 View
We are trying to measure degree of conversion of resin composite under ceramic restoration using FTIR. The specimens are flat and are cured next to a cellophane strip, so they are smooth and...
01 November 2023 8,494 1 View
I am having trouble understanding the different approaches where pull down vs co IP is used. I have to study protein- protein interactions associated with a specific protein. I have developed an...
31 October 2023 2,733 4 View
Hi all, I am simulating a flow over a cylinder using LES. Looking at several academic papers on the same topic, statistics are calculated after several vortex shedding cycles. I would like to...
30 October 2023 740 7 View
I've been looking into 1-3 piezoelectric composites lately. I am particularly interested in the process of making them with high aspect ratio. However, I have come across conflicting opinions in...
30 October 2023 4,913 0 View
I am trying to minimize the gate leakage current of a FET devices and found that hBN is a good dielectric for 2D materials (MoS2, WS2) based FETs. I was curious if we can deposite it by e beam...
30 October 2023 7,248 0 View
I work in a project where we need to knockout a gene at primary T cells targeting an exon that is shared between all variants. However, while the sgRNA design has been optimised ,we still see low...
25 October 2023 5,744 1 View
Hello, we need to sterilize borosilicate made transparent glass vials with gamma radiation (15 or 25kgray). Question 1: Do these glasses discolor after radiation? Question 2: Does this...
24 October 2023 1,769 0 View
Made a lot of CsI films with Tl doping by vacuum evapration, did SEM and ICP and other optical test, but never find out what is the key effect of CsI afterglow. Need some suggestion for next step.
23 October 2023 5,227 0 View
Dear all, We have a landfill which is filled with sludge containing high amount of Arsenic (approximately 412,000 ppm). This sludge is the byproduct of a acid factory wastewater treatment plant...
16 October 2023 9,942 2 View
Hi, I am a final-year Psychology student at Newcastle University and I would like to explore the concept of women being addicted to pregnancy. I would ideally like to create a report on this for...
13 October 2023 2,501 5 View
We want to analyze the heavy metals (Cd, Pb and Cr) in aqueous solution by using UV-VIS spectroscopy. The concentration range is 10-150ppm.
12 October 2023 3,148 3 View
was thinking of a combination of MoCl5 and PCl3, but curious if someone with more experience in TMs/inorganic chemistry would be able to shed some light. thank you
12 October 2023 8,271 1 View
Currently, I am working on mouse or rat models' primary culture of liver cells (particularly hepatocytes). In most of the previous research, I found the protocol stressing the perfusion of the...
11 October 2023 3,711 0 View
Hello I want the names of researchers in the field "entropy generation of fluid flow through" channel
10 October 2023 7,328 0 View
In the beginning after plating, I could not get colonies, then later I started having colonies streaked and there were nice isolated single colonies. After that, I prepared a miniprep but there...
05 October 2023 2,021 0 View
Hello Everyone! I hope you all are doing well! I am trying to do some analytical calculations for the hydrogen absorption and was wondering if: Q =m delta H...
04 October 2023 2,403 4 View
Hello. We are writing a master thesis on microstructures of composite materials and we want to combine the cohesive zone model (CZM) for delamination of fibres and the phase-field model (PFM) for...
04 October 2023 5,648 4 View
Dear All, I want to kindly ask a question about enzymatic hydrolysis of plant based proteins. We have proteases as Flavourzyme and we hydrolyze pea and wheat mixed protein. We construct a process...
02 October 2023 1,070 1 View
Hi, I am working on grid connected PV inverters for high power rating (> 1 MW). Many transmissions companies now requesting the PV farms to support reactive power during night time. I am...
27 September 2023 941 0 View
Do you know any laboratory within the Philippines that processes fish identification via DNA barcoding using mitochondrial COI as a marker?
27 September 2023 1,655 0 View
For example We have 10ul RBCs and want to know the total number of Antigen X on 10ul RBCs.
25 September 2023 1,522 2 View