Dear Scientists, I have noticed that some stem cell companies are promoting the use of UCMSC/WJMSC in passage 2 and claiming that it is superior to passages 3-5, which are commonly used in clinical trials. As someone working in this field, I am skeptical about being able to validate the specific passage number through routine tests. Most clinical trials have utilized passages 3-5 with positive outcomes.
My question is, which passage is the safest option? Are there significant advantages to using passage 2 compared to the latest passages? I appreciate your response in advance.
Generally, cells at lower passages may have higher proliferative capacity and exhibit more robust stem cell properties. However, there is a risk of genetic instability and accumulation of mutations with prolonged culture, which can increase with higher passage numbers. Therefore, cells at earlier passages may be considered safer in terms of genomic stability.
Thanks for your answer. My question is a comparison between P2 and P4.
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